sibvestСибирский вестник сельскохозяйственной наукиSiberian Herald of Agricultural Science0370-87992658-462XSiberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences10.26898/0370-8799-2022-3-8sibvest-1073Research ArticleЗООТЕХНИЯ И ВЕТЕРИНАРИЯZOOTECHNICS AND VETERINARY MEDICINEВлияние преобразованной лузги подсолнечника на ферментативные процессы в рубце жвачных животных in vitroEffect of transformed sunflower husk on enzymatic processes in the rumen in vitroМирошниковС. А.MiroshnikovS. A.

член-корреспондент, РАН, научный сотрудник,

Оренбург

Corresponding Member RAS, Researcher,

 

ДускаевГ. К.DuskaevG. K.

доктор биологических наук, заместитель директора, ведущий научный сотрудник,

Оренбург

Doctor of Science in Biology, Deputy Director, Lead Researcher,

 

ШейдаЕ. В.SheidaE. V.

научный сотрудник, кандидат биологических наук,

Оренбург

Researcher, Candidate of Science in Biology,

 

РязановВ. А.RyazanovV. A.

кандидат сельскохозяйственных наук, научный сотрудник,

460000, Оренбург, ул. 9 января, д. 29

Candidate of Sciences in Agriculture, Researcher,

29, 9th January St., Orenburg, 460000

vita7456@yandex.ruФедеральный научный центр биологических систем и агротехнологий Российской академии наукРоссияFederal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of SciencesRussian Federation2022260720225237178Copyright © Мирошников С.А., Дускаев Г.К., Шейда Е.В., Рязанов В.А., 20222022Мирошников С.А., Дускаев Г.К., Шейда Е.В., Рязанов В.А.Miroshnikov S.A., Duskaev G.K., Sheida E.V., Ryazanov V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://sibvest.elpub.ru/jour/article/view/1073

Изучены процессы ферментации в рубце жвачных при использовании лузги подсолнечника, подвергнутой ультразвуковой обработке, в сочетании с фитобиотиками. В ходе эксперимента определяли образование конечных метаболитов в рубцовой жидкости, а также образование метана. Объектом исследования являлась рубцовая жидкость, которая отобрана у бычков казахской белоголовой породы в возрасте 12 мес средней массой 230–235 кг. Хроническая фистула рубца (n = 3) была установлена по методике А.А. Алиева. Методом in vitro при помощи прибора «ANKOM Daisy II» (модификации D200 и D200I) по специализированной методике проинкубированы следующие образцы: контроль (образец № 1): лузга, подвергнутая механическому измельчению, + гидромодуль (вода) + обработка УЗ при 20 °С, 15 мин, 27 кГц; образец № 2: образец № 1 + гамма-окталактон (0,25 мл); образец № 3: образец № 1 + кверцетин (10,0 мг); образец № 4: образец № 1 + 7-гидроксикумарин (2,0 мг). Каждый эксперимент проведен в пяти повторностях. Определены переваримость сухого вещества, концентрация летучих жирных кислот, образование азотистых метаболитов и концентрация метана. Полученные данные статистически проанализированы с использованием программного обеспечения. Получены новые данные о влиянии лузги подсолнечника совместно с фитобиотиками на образование конечных метаболитов в рубцовой жидкости. Установлено, что добавление биологически активных веществ гамма-окталактона, кверцетина, 7-гидроксикумарина способствовало повышению переваримости сухого вещества относительно контроля на 2,0% (р ≤ 0,05), 3,1 (р ≤ 0,01) и 4,3% (р ≤ 0,05) соответственно. Отмечено повышение концентрации летучих жирных кислот и азотистых фракций при использовании данных веществ. Уровень концентрации метана был ниже, чем в контроле, при использовании 7-гидроксикумарина в образце № 4 на 10% (р ≤ 0,01). 

Fermentation processes in the rumen of ruminants when using sunflower husk subjected to ultrasound treatment in combination with phytobiotics were studied. During the experiment, the formation of terminal metabolites in the ruminal fluid as well as the formation of methane were determined. The object of the study was the rumen fluid, which was selected from bulls of Kazakh whiteheaded breed at the age of 12 months with the average weight of 230-235 kg. Chronic rumen fistula (n = 3) was identified according to the method of A.A. Aliev. The following samples were incubated by in vitro method using the ANKOM Daisy II device (modifications D200 and D200I) according to a specialized technique: control (sample N 1): Mechanically ground husk + hydromodulus (water) + ultrasound treatment at 20 °C, 15 min, 27 kHz; sample N 2: sample N 1 + gamma-octalactone (0.25 ml); sample N 3: sample N 1 + quercetin (10.0 mg); sample N 4: sample N 1 + 7-hydroxycoumarin (2.0 mg). Each experiment was conducted in five replications. The digestibility of dry matter, concentration of volatile fatty acids, formation of nitrogenous metabolites and methane concentration were determined. The data obtained were statistically analyzed using software. New data were obtained on the effect of sunflower husk together with phytobiotics on the formation of final metabolites in the rumen fluid. The addition of biologically active substances of gamma-octalactone, quercetin, 7-hydroxycoumarin was found to increase the digestibility of dry matter relative to control by 2.0 (p ≤ 0.05), 3.1 (p ≤ 0.01) and 4.3% (p ≤ 0.05), respectively. An increase in the concentration of volatile fatty acids and nitrogenous fractions was noted when using these substances. The level of methane concentration was 10% lower than that of the control with 7-hydroxycoumarin in sample N 4 (p ≤ 0.01). 

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The authors declare that there are no conflicts of interest present.