sibvestСибирский вестник сельскохозяйственной наукиSiberian Herald of Agricultural Science0370-87992658-462XSiberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences10.26898/0370-8799-2023-6-4sibvest-1461Research ArticleРАСТЕНИЕВОДСТВО И СЕЛЕКЦИЯPLANT GROWING AND BREEDINGАнализ активности окислительных ферментов методом многомерной регрессии в присутствии Mg2+ мицелия гриба вешенкаAnalysis of the activity of oxidative enzymes by multivariate regression in the presence of Mg2+ oyster mushroom myceliumЛовцоваЛ. Г.LovtsovaL. G.

кандидат технических наук, доцент;410012, Саратов, Театральная площадь, 1

Candidate of Science in Engineering, Associate Professor

larisalovtsova2018@mail.ruЗабелинаМ. В.ZabelinaM. V.

доктор биологических наук, профессор

Doctor of Science in Biology, Professor

МайоровА. В.MayorovA. V.

младший научный сотрудник

 Junior Researcher

УсковК. Ю.UskovK. Yu.

аспирант

Post-graduate Student

ТюринИ. Ю.TyurinI. Yu.

кандидат технических наук, доцент

Candidate of Science in Engineering, Associate Professor

МавзовинВ. С.MavzovinV. S.

кандидат математических наук, доцент

Candidate of Science in Mathematics, Associate Professor

Саратовский государственный университет генетики, биотехнологии и инженерии имени Н.И. ВавиловаРоссияSaratov State University of Genetics, Biotechnology and Engineering named after N.I. VavilovRussian FederationМосковский государственный строительный университетРоссияMoscow State University of Civil EngineeringRussian Federation2023030820235363342Copyright © Ловцова Л.Г., Забелина М.В., Майоров А.В., Усков К.Ю., Тюрин И.Ю., Мавзовин В.С., 20232023Ловцова Л.Г., Забелина М.В., Майоров А.В., Усков К.Ю., Тюрин И.Ю., Мавзовин В.С.Lovtsova L.G., Zabelina M.V., Mayorov A.V., Uskov K.Y., Tyurin I.Y., Mavzovin V.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://sibvest.elpub.ru/jour/article/view/1461

Проведены исследования по оптимизации процесса глубинного культивирования мицелия гриба вешенка. Усовершенствован процесс получения мицелия как посевного материала для выращивания плодовых тел грибов. Изучено влияние различных концентраций карбоната магния (Magnesium carbonates) на ростовые характеристики мицелия гриба Pleurotus ostreatus при глубинном культивировании. Выявлена зависимость активности ферментов от концентрации металла в питательной среде прорастания мицелия вешенки. Адаптирована методика определения активности каталазы спектрофотометрическим методом для изучаемых объектов. Впервые получены данные активности ферментов каталазы и супероксиддисмутазы мицелия в присутствии добавки карбоната магния. Установлено, что применение карбоната магния в малых концентрациях положительно влияет на рост биомассы мицелия гриба Pleurotus ostreatus, поскольку с увеличением концентрации Mg2+ отмечено уменьшение скорости роста биомассы и активности каталазы, предположительно, за счет участия магния в создании определенной ионной концентрации, при которой начинается инактивация каталазы. Изучена возможность применения метода многомерной регрессии в виде метода главных компонент (МГК). Проведен анализ редокс-состояния культуры Pleurotus ostreatus на уровне ферментных компонентов системы антиоксидантной защиты при погруженном культивировании базидомицетов, который показал, как взаимосвязаны между собой полученные переменные с разными единицами измерений. Графики счетов также наглядно указывают на зависимость роста мицелия от концентрации применяемой добавки. Введение предложенных в работе условий культивирования в практику грибоводства потенциально способствует более успешному противостоянию макромицетов биотическому и абиотическому стрессу. Результаты исследований актуальны для развития фундаментальных основ науки о грибах.

Studies have been conducted to optimize the process of deep cultivation of the Oyster mushroom mycelium. The process of obtaining mycelium as a seed for cultivation of fruiting bodies of mushrooms has been improved. The effect of different concentrations of magnesium carbonate (Magnesium carbonates) on the growth characteristics of mycelium of the fungus Pleurotus ostreatus during deep cultivation has been studied. The dependence of enzyme activity on the concentration of metal in the nutrient medium of germinating mycelium of oyster mushrooms has been revealed. The method for determining the activity of catalase by spectrophotometric method has been adapted for the studied objects. For the first time the data on the activity of mycelium catalase and superoxide dismutase enzymes in the presence of magnesium carbonate additive have been obtained. It has been found that the application of magnesium carbonate in low concentrations has a positive effect on the growth of mycelial biomass of the fungus Pleurotus ostreatus, since with increasing concentration (Mg2+) a decrease in biomass growth rate and catalase activity has been observed, presumably due to the participation of magnesium in creating a certain ionic concentration at which catalase inactivation begins. The possibility of applying the method of multivariate regression in the form of the principal components analysis (PCA) has been studied. The redox state of Pleurotus ostreatus culture at the level of enzyme components of the antioxidant defense system during submerged cultivation of basidomycetes has been analyzed, which showed how the obtained variables with different measurement units are interconnected. The account graphs also clearly indicate the dependence of mycelial growth on the concentration of the additive used. The introduction of the cultivation conditions proposed in this work in the practice of mushroom production potentially contributes to a more successful resistance of macromycetes to biotic and abiotic stress. The results of the research are relevant to the development of the fundamentals of the science of fungi.

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The authors declare that there are no conflicts of interest present.