sibvestСибирский вестник сельскохозяйственной наукиSiberian Herald of Agricultural Science0370-87992658-462XSiberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences10.26898/0370-8799-2023-10-3sibvest-1593Research ArticleРАСТЕНИЕВОДСТВО И СЕЛЕКЦИЯPLANT GROWING AND BREEDINGОсобенности размножения гибридов клюквы крупноплодной (Oxycoccus macrocarpus) в культуре in vitroFeatures of large cranberry (Oxycoccus macrocarpus) hybrids propagation in in vitro cultureЭрстА. А.ErstA. A.

кандидат биологических наук, старший научный сотрудник

630090, Новосибирск, ул. Золотодолинская, 101

Anna A. Erst, Candidate of Science in Biology, Senior Researcher

101, Zolotodolinskaya St., Novosibirsk, 630090

annaerst@yandex.ruГорбуновА. Б.GorbunovA. B.

кандидат биологических наук, старший научный сотрудник

Новосибирск

Aleksey B. Gorbunov, Candidate of Science in Biology, Senior Researcher

Novosibirsk

 

ТитовЕ. В.TitovE. V.

инженер

Новосибирск

Evgeny V. Titov, Engineer

Novosibirsk

Центральный сибирский ботанический сад Сибирского отделения Российской академии наукРоссияCentral Siberian Botanical Garden of the Siberian Branch of the Russian Academy of SciencesRussian Federation20232011202353102330Copyright © Эрст А.А., Горбунов А.Б., Титов Е.В., 20232023Эрст А.А., Горбунов А.Б., Титов Е.В.Erst A.A., Gorbunov A.B., Titov E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://sibvest.elpub.ru/jour/article/view/1593

Настоящее исследование направлено на разработку системы микроразмножения пяти межсортовых гибридов Oxycoccus macrocarpus, перспективных для выращивания на территории Западной Сибири. Все исследования по созданию методики размножения in vitro проведены на примере межсортового гибрида O. macrocarpus Bergman × Pilgrim. Гибриды Ben Lear × Bergman, Ben Lear × Pilgrim, Pilgrim × Bergman и Pilgrim × Ben Lear размножены in vitro по разработанной для Bergman × Pilgrim технологии. Всхожесть семян гибридов в культуре in vitro составила от 35,29 до 80,00%. На этапе массового размножения установлено, что концентрация 2-изопентиладенина 0–10 мкМ не влияет на коэффициент размножения. Тестируемые значения рН (4,5–7,5) не вызывали гибель гибрида Bergman × Pilgrim, что указывает на его устойчивость в данном диапазоне рН. Отмечено активное корнеобразование на средах с цитокинином (100,0%), в связи с чем отдельной стадии укоренения микропобегов не требовалось. На этапе адаптации к условиям ex vitro установлено, что использование в качестве субстрата сфагнового мха является эффективным приемом. Жизнеспособность растений составила 86,0–93,0%. Показаны достоверные различия после этапа адаптации: по количеству побегов – между гибридами Bergman × Pilgrim (1,21 шт./побег) и Ben Lear × Bergman (7,00 шт./побег), по длине корней – между Bergman × Pilgrim (19,7 мм), Ben Lear × Bergman (39,4 мм) и Pilgrim × Bergman (53,4 мм). Применение методов in vitro для размножения и отбора генотипов является эффективным подходом и может применяться в программах получения межсортовых гибридов O. macrocarpus.

The present study is aimed at developing a micropropagation system for five intervarietal hybrids of Oxycoccus macrocarpus promising for cultivation in Western Siberia. All studies on the development of in vitro propagation techniques were carried out on the example of the intervarietal hybrid О. macrocarpus Bergman × Pilgrim. Ben Lear × Bergman, Ben Lear × Pilgrim, Pilgrim × Bergman, and Pilgrim × Ben Lear hybrids were propagated in vitro using the technology developed for Bergman × Pilgrim. Seed germination of the hybrids in in vitro culture ranged from 35.29 to 80.00%. At the mass reproduction stage, it was found that concentrations of 2-isopentyl adenine 0–10 μM did not affect the reproduction rate. The tested pH values (4.5–7.5) did not cause the death of Bergman × Pilgrim hybrid, indicating its stability in this pH range. Active root formation on the media with cytokinin (100.0%) was observed, and therefore a separate stage of rooting of microshoots was not required. During the ex vitro adaptation phase, it was found that the use of sphagnum moss as a substrate served as an effective technique. The plant viability was 86.0–93.0%. Significant differences after the adaptation stage were shown: in shoot number between Bergman × Pilgrim (1.21 pcs./shoot) and Ben Lear × Bergman (7.00 pcs./shoot) hybrids, in root length between Bergman × Pilgrim (19.7 mm), Ben Lear × Bergman (39.4 mm) and Pilgrim × Bergman (53.4 mm). The use of in vitro methods for propagation and selection of genotypes is an effective approach and can be applied in programs to obtain intervarietal hybrids of O. macrocarpus.

клюквагибридыразмножение in vitropH питательной средыcranberryhybridsin vitro propagationnutrient medium pHРабота выполнена в рамках государственного задания ФГБУН «Центральный сибирский ботанический сад Сибирского отделения Российской академии наук», проекты № АААА-А21-121011290027-6 и АААА-А21-121011290025-2. При подготовке публикации использованы материалы биоресурсной научной коллекции Центрального сибирского ботанического сада «Коллекции живых растений в открытом и закрытом грунте», УНУ № USI 440534.The work was carried out under the state assignment of the FSBIS "Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences", projects No. AAAA-A21-121011290027-6 and AAAA-A21-121011290025-2. 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The authors declare that there are no conflicts of interest present.