sibvestСибирский вестник сельскохозяйственной наукиSiberian Herald of Agricultural Science0370-87992658-462XSiberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences10.26898/0370-8799-2020-1-6sibvest-652Research ArticleЖИВОТНОВОДСТВО И ВЕТЕРИНАРИЯANIMAL HUSBANDRY ABD VETERINARY SCIENCEФенотипические и молекулярно-генетические свойства Microsporum canisPhenotypic and molecular genetic properties of Microsporum canisКухарЕ. В.KukharE. V.

доктор биологических наук, доцент

г. Нур-Султан

Doctor of Science in Biology, Assistant Professor

kucharev@mail.ruКиянВ. С.KiyanV. S.

доктор PhD, руководитель

г. Нур-Султан

PhD, Head

vskiyan@gmail.comГлотоваТ. И.GlotovaT. I.

доктор биологических наук, профессор, главный научный сотрудник

630501, Новосибирская область, р.п. Краснообск; СФНЦАРАН, а/я 463

Doctor of Science in Biology, Professor, Head Researcher

PO Box 463, SFSCA RAS, Krasnoobsk, Novosibirsk Region, 630501

t-glotova@mail.ruГлотовА. Г.GlotovA. G.

доктор ветеринарных наук, профессор, заведующий лабораторией

Новосибирская область, р.п. Краснообск

Doctor of Science in Veterinary Medicine, Professor, Head of the Laboratory

Krasnoobsk, Novosibirsk region

glotov_vet@mail.ruКазахский агротехнический университет им. С. СейфуллинаКазахстанS. Seifullin Kazakh AgroTechnical University Nur-SultanKazakhstanСибирский федеральный научный центр агробиотехнологий Российской академии наукРоссияSiberian Federal Scientific Centre of AgroBioTechnologies of the Russian Academy of SciencesRussian Federation2020260320205014856Copyright © Кухар Е.В., Киян В.С., Глотова Т.И., Глотов А.Г., 20202020Кухар Е.В., Киян В.С., Глотова Т.И., Глотов А.Г.Kukhar E.V., Kiyan V.S., Glotova T.I., Glotov A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://sibvest.elpub.ru/jour/article/view/652

Проведены многолетние исследования (20122018) культур микроскопических грибов (дерматомицетов), выделенных от клинически больных кошек. Отбор проб биоматериала осуществлен от домашних животных с признаками поражения кожи и шерстного покрова в ветеринарных клиниках Республики Казахстан. Молекулярногенетические исследования, видовая идентификация и определение свойств выделенных культур проведены с помощью утвержденных методических рекомендаций и определителей патогенных и условно-патогенных грибов. Изучены культурально-морфологические (фенотипические), кератинолитические, биохимические и молекулярно-генетические свойства основного вида микроскопических грибов, вызывающего дерматомикозы, – Microsporum canis. В результате исследований получены следующие четыре штамма гриба Microsporum canis: 13 Kz, 376 Kz, 384 Kz и 428 Kz. Изученные штаммы характеризовались разнообразием фенотипических свойств (формирование разных по морфологии колоний на питательных средах, разнообразная окраска воздушного и субстратного мицелия), сходством микроструктур (септированный бамбукообразный мицелий с характерным ветвлением, двухслойные веретенообразные макроконидии), биохимических свойств (наличие высокой сахаролитической и уреазной активности) и кератинолитической активностью. Фенотипические характеристики полностью соответствовали культуре микроскопического гриба Microsporum canis. Молекулярногенетические исследования установили идентичность последовательностей полученных штаммов гриба Microsporum canis 13 Kz, 376 Kz, 384 Kz и 428 Kz с опубликованными в GenBank последовательностями штаммов EU181444.1, EF581130.1 и EF581129.1. Молекулярно-генетические исследования позволили идентифицировать полученные культуры дерматомицетов как анаморфную стадию Microsporum canis, а также как стадию телеоморфа (Arthroderma otae).

Long-term studies (2012–2018) of cultures of microscopic fungi (dermatomycetes) isolated from clinically sick cats were carried out. Samples of biomaterial were taken from domestic animals with signs of skin and coat damage in veterinary clinics of the Republic of Kazakhstan. Molecular genetic studies, species identification and determination of the properties of selected cultures were carried out in accordance with the approved methodological recommendations and with the use of determinants of pathogenic and potentially pathogenic fungi. Cultural-morphological (phenotypic), keratinolytic, biochemical and molecular genetic properties of the main species of microscopic fungi that cause dermatomycosis – Microsporum canis were studied. As a result of research, four strains of the fungus Microsporum canis, 13 Kz, 376 Kz, 384 Kz, and 428 Kz, were obtained. The studied strains were characterized by a variety of phenotypic properties (they formed colonies with different morphology on nutrient media and had a different color of air and substrate mycelium), as well as similar microstructures (they had septate bamboolike mycelium with characteristic branching, bilayer spindle-shaped macroconidia), biochemical properties (the presence of high saccharolytic and urease activity) and keratinolytic activity. Phenotypic characteristics fully corresponded to the culture of the microscopic fungus Microsporum canis. Molecular genetic studies established the identity of the sequences of the obtained strains of the fungus Microsporum canis 13 Kz, 376 Kz, 384 Kz and 428 Kz with the sequences of strains EU181444.1, EF581130.1 and EF581129.1 published in GenBank. Molecular genetic studies made it possible to identify the cultures of dermatomycetes that were obtained as an anamorphic stage of Microsporum canis, as well as a teleomorph known as Arthroderma otae.

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The authors declare that there are no conflicts of interest present.