THE RAPID METHOD FOR MASS DIAGNOSIS OF BRUCELLOSIS IN ANIMALS BASED ON ELISA
Abstract
A new ELISA test system for diagnosis of brucellosis in animals has been developed. Results obtained from studies demonstrate that this test system can be employed in many animal species, including sheep and cattle, even when used live vaccines against brucellosis derived from weakly agglutinogenic and agglutinogenic strains. It has been found that the new ELISA test system absorbs readings of the indirect agglutination test, relating to rapid methods, as well as of formal classical diagnostic complex (agglutination test, AT, + complement fixation test, CFT), is not inferior to them in specificity and superior to them in diagnostic activity. Advantages have been revealed associated with reducing time for research, recording and interpretation of results, with simplifying these processes and increasing their objectivity, reproducibility and safety. Introduction of the new test system to practice of screening tests for brucellosis was proved to greatly simplify mass diagnosis of this disease, since the AT+CFT complex and additional differential postvaccinal diagnosis should be employed only in a case of detecting positive or doubtful readings.
About the Authors
A. S. Dimova
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
A. А. Sizov
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
S. K. Dimov
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
G. M. Stebleva
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
N. I. Kurenskaya
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
D. A. Sizov
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
D. P. Melnikov
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
P. K. Arakelyan
All-Russian Research Institute of Brucellosis and Tuberculosis in Animals, Russian Academy of Agricultural Sciences
Russian Federation
Russian Federation
A. A. Gritsan
Tomsk Regional Veterinary Laboratory
Russian Federation
Russian Federation
I. N. Emelyanova
Tomsk Regional Veterinary Laboratory
Russian Federation
Russian Federation
References
1. Косилов И.А., Аракелян П.К., Димов С.К., Хлыстунов А.Г. Бруцеллез сельскохозяйственных животных / под ред. И.А. Косилова. - Новосибирск, 1999. - 344 с.
2. Каликин И.Н., Дегтяренко Л.В., Карлова М.Ю., Скляров О.Д. Усовершенствование серологической экспресс-диагностики бруцеллеза животных // Сиб. вестн. с.-х. науки. - 2012. - № 1. - С. 93-98.
3. Хаиров С.Г. РНГА в диагностике бруцеллеза крупного рогатого скота / Вестн. ветеринарии. - 2005. - Т. 32. - С. 18-26.
4. Ромахов Б.Н., Малышева Л.А., Руденко В.П. Испытание РНГА с эритроцитарным антигеном для диагностики бруцеллеза крупного рогатого скота и овец // Вет. патология. - 2008. № 1. - С. 73-76.
5. Пат. № 2152035 от 27.06.2000 г. (Российская Федерация). Способ диагностики бруцеллеза / З.Ф. Богаутдинов, Е.С. Вылегжанина, В.Б. Кузьмина, Т.С. Астахова, У.Э. Ниязов, К.В.Шумилов. - Номер заявки 99117990/13; заявл. 18.08. 1999; опубл. 27.06.2000; Бюл. № 18.
6. Пат.№2203499 от 27.04.2003 г. (Российская Федерация). Способ постановки диагностической реакции при бруцеллезе / П.Е. Игнатов, А.И.Федоров, Л.П. Мельниченко. - Номер заявки 2001124871/13; заявл. 12.09. 2001; опубл. 27. 04. 2003.; Бюл. № 12.
Review
For citations:
Dimova A.S., Sizov A.А., Dimov S.K., Stebleva G.M., Kurenskaya N.I., Sizov D.A., Melnikov D.P., Arakelyan P.K., Gritsan A.A., Emelyanova I.N. THE RAPID METHOD FOR MASS DIAGNOSIS OF BRUCELLOSIS IN ANIMALS BASED ON ELISA. Siberian Herald of Agricultural Science. 2014;(4):84-90. (In Russ.)
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