Study of the effects of butyric acid and propanediol of the intestine in ICR mice

The effect of gut microbiota correctors on the motility of the gastrointestinal tract of ICR mice was studied. Two feed additives were used - compositions based on glycerol and 1.3 propanediol and Salkoli Mono BP Dry. Three groups of 10-12 animals each were formed according to the principle of analogues: two experimental and one control. Experimental feed mixtures were made by saturating 200 g of feed with sunflower oil, 1.3 propanediol, glycerol (experimental group 1); the feed mixture of experimental group 2 was additionally mixed with the feed additive Salkoli Mono BP Dry. To estimate the rate of fecal excretion, 100 µl of fluorescent tags (fluorescent ink green and red) were given to mice of all groups, previously mixed with water 1: 1. The timing of the appearance of the first signs of fluorescence in feces in animals of the control and experimental groups was estimated. Intensive fluorescence of the green label in fluorimetry samples was observed. The greatest increase in fluorescence was observed at the 4th hour of the experiment in the control group, while in the 2nd experimental group the increase lasted until the 5th hour. In the 1st experimental group, elimination of the main amount of green dye did not begin even by the 5th hour of the experiment. The method using fluorescent tags revealed a delay in mixing of feed masses in mice fed glycerol and 1,3 propanediol, which was accompanied by a statistically significant increase in Escherichia coli concentration - by 3 times (p < 0.05). The use of Salkoli Mono BP Dry had no statistically significant effect on E. coli concentrations and gastrointestinal motility.

1. Kubota M., Ito K., Tomimoto K. Lactobacillus reuteri DSM 17938 and Magnesium Oxide in Children with Functional Chronic Constipation: A Double-Blind and Randomized Clinical Trial. Nutrients, 2020, no. 12 (1), рр. 225. DOI:10.3390/nu12010225.

2. Komarova O.N. Effectiveness of Lactobacillus reuteri application in clinical practice. RMZh. Mat' i ditya = Russian Medical Journal. Mother and Child, 2021, no. 4 (3), pp. 277–283. (In Russian). DOI:10.32364/2618-8430-2021-4-3-277-283.

3. Mota M.J., LopesR.P., Sousa S., Gomes A.M., Delgadillo I., Saraiva J.A. Lactobacillus reuteri growth and fermentation under high pressure towards the production of 1,3-propanediol. Food research international, 2018, no. 113, pp. 424–432. DOI:10.1016/j.foodres.2018.07.034.

4. Semenikhina V.F., Rozhkova I.V., Begunova A.V., Fedorova T.V., Shirshova T.I. Development of biotechnology of fermented milk product with Lactobacillus reuteri LR1 and the evaluation of its functional property in e4xperiment in vitro and in vivo. Voprosy pitaniya = Problems of Nutrition, 2018, no. 87 (5), pp. 52–62. (In Russian). DOI:10.24411/0042-8833-2018-10053.

5. Afonyushkin V.N., Khomenko Yu.S., Frolova O.A., Kozlova Yu.N., Sigareva N.A. The analysis of planning system for antisalmonella measures on a poultry plant. Ptitsa i ptitseprodukty = Poultry and Chicken Products, 2019, no. 3, pp. 20–23. (In Russian). DOI:10.30975/2073-4999-2019-21-3-20-23.

6. Begunova A.V., Rozhkova I.V., Shirshova T.I., Glazunova O.A., Fedorova T.V. Optimization of conditions for Lactobacillus reuteri LR1 strain cultivation to improve the biosynthesis of bacteriocin-like substances. Biotekhnologiya = Biotechnology in Russia, 2019, no. 5 (35), pp. 58–69. (In Russian). DOI:10.21519/0234-2758-2019-35-5-58-69.

7. Zakharova I.N., Berezhnaya I.V., Sugyan N.G., Sannikova T.N., Kuchina A.E., Sazanova Yu.O. What do we know today about Lactobacillus reuteri? Meditsinskiy Sovet = Medical Council, 2018, no. 2, pp. 163–169. (In Russian). DOI:10.21518/2079-701X-2018-2-163-169.

8. Saviano A., Brigida M., Migneco A., Gunawardena G., Zanza C., Candelli M., Franceschi F., Ojetti V. Lactobacillus reuteri DSM 17938 (Limosilactobacillus reuteri) in Diarrhea and Constipation: Two Sides of the Same Coin? Medicina, 2021, no. 57. DOI:10.3390/medicina57070643.

9. Afonyushkin V.N., Davydova N.V., Tromenshleger I.N., Mishukova O.V., Kozlova Yu.N., Cherepushkina V.S., Mironova T.E., Klemeshova I.Yu. Salmonella infection level in chicken populations versus antagonistic activity of Lactobacillaceae and Enterococcaceae against Salmonella enterica. Vestnik NGAU = Bulletin of NSAU, 2020, no. 1, pp. 48–55. (In Russian). DOI:10.31677/2072-6724-2020-54-1-48-55.

10. Ong T.G., Gordon M., Banks S.S., Thomas M.R., Akobeng A.K. Probiotics to prevent infantile colic. Cochrane Database Syst Rev, 2019, no. 133 (3). DOI:10.1002/14651858.CD012473.pub2.