ANTIMICROBIAL ACTION OF PHOTOSENSITIZER METHYLENE BLUE ON PARAMECIUM CAUDATUM
Abstract
There was studied the action of photosensitizer methylene blue in concentrations of 0.02, 0.1, and 1.0%, and exposure time of optical radiation of 15 minutes on the protozoa Paramecium caudatum. A light-emitting diode with output power of 3500 milliwatts and wavelength of 620 nanometers was used as a light source. In the first experiment, methylene blue dye solution in the concentrations stated above was added to paramecium suspension in the same proportion; optical irradiation was not performed; mobility of paramecia and time to death were evaluated. It was found that survival times of paramecia made up 15, 10, and 3 minutes as influenced by photosensitizer solutions in concentrations of 0.02, 0.1, and 1.0%, respectively. In the control group, mobility of paramecia did not change. In the second experiment, paramecia were irradiated for 15 minutes. The control group of paramecia was irradiated without photosensitizer. Survival times of paramecia in the second experiment made up 5, 2, and 1 minutes in solutions with photosensitizer concentrations of 0.02, 0.1, and 1.0%, respectively. Mobility of paramecia of the control group did not change. It has been found that the impact of optical radiation on the photosensitizer solution 2-5 times accelerates the death of paramecia, and the magnitude of the antimicrobial action is in direct proportion to the solution concentration and exposure time of optical radiation. This method is promising for the treatment of infectious diseases caused by antibiotic-resistant microorganisms.
About the Authors
A. V. Pavlov
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
E. YU. Smertina
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
YU. G. Yushkov
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
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Review
For citations:
Pavlov A.V., Smertina E.Yu., Yushkov Yu.G. ANTIMICROBIAL ACTION OF PHOTOSENSITIZER METHYLENE BLUE ON PARAMECIUM CAUDATUM. Siberian Herald of Agricultural Science. 2014;(6):77-81. (In Russ.)
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