Associations of somatotropin (GH) gene polymorphism with lactation performance of cows

The research was conducted in the laboratory of molecular genetic expertise in 2018 and 2019 within the framework of the state assignment on the topic "Development of genetic-selection and theoretical foundations for the conservation and effective use of the gene pool of cattle in the Ural region with the use of modern biotechnologies". The object of the study is the cattle of Holsteinized black-and-white breed. The main task is to analyze the degree of influence of the somatotropin (GH) gene polymorphism on lactation performance indicators. In the course of the study, DNA from 270 cows was isolated, the polymorphism of the somatotropin gene was determined based on the obtained data, and the relationship between different genotypes and indicators of live weight, milk yield, protein mass fraction and fat mass fraction in milk was established. The results were biometrically processed using IBM SPSS Statistics 23 software program. It was revealed that LL is the main genotype of the GH gene among the representatives of the Holsteinized black-and-white breed (frequency of occurrence 77.0%). The proportion of LV and VV genotypes in the population is 21.9 and 1.1%, respectively. During the observation period, cows carrying the LL genotype showed higher milk yields in the first and third lactations. The difference with their peers was 202-2334 kg (p ≤ 0.05). A tendency towards increased protein content in milk was recorded in animals with the VV genotype. In the first lactation, the difference with LL and LV genotype carriers was 0.03 and 0.06%, respectively. In the third lactation, individuals with the VV genotype surpassed their female counterparts in protein content by 0.07 and 0.12%, respectively.

1. Bagal E.I., Pavlova I.Yu., Khabibrakhmanova Ya.A., Kalashnikova L.A., Yaluga V.L. Genotyping Kholmogor and Holstein cattle in the genes of prolactin and somatotropin. Vestnik Rossiyskoy akademii sel′skokhoziaystvennih nauk = Vestnik of the Russian Agricultural Science, 2014, no. 5, pp. 11–13. (In Russian).

2. Bobryshova G.T., Surzhikova E.S., Chudnovets A.I. Polymorphism of the growth hormone gene (GH) and its relationship with the productive qualities of cows of the Yaroslavl breed. Glavniy zootekhnik = Head of Animal Breeding, 2019, no. 12, pp. 31–37. (In Russian).

3. Nekrasov D.K., Kolganov A.E., Kalashnikova L.A., Semashkin A.V. The relationship of polymorphic variants of genes of the prolactin, growth hormone and kappa-casein with milk productivity of cows of the Yaroslavl breed. Agrarniy vestnik Verkhnevolzhiya = Agrarian journal of Upper Volga region, 2017, no. 1 (18), pp. 40–48. (In Russian).

4. Dzhulamanov K.M., Gerasimov N.P., Dubovskova M.P., Baktygalieva A.T. Polymorphisms of CAPN1, CAST, GDF5, TG5 and GH genes in Russian Hereford cattle. Bulgarian journal of agricultural science, 2019, no. 25 (23), pp. 75– 79.

5. Kopylov K.V., Biryukova O.D., Berezovsky A.V., Basovsky D.N. The genetic monitoring in the herd of the Ukrainian Redand-White dairy breed on gene complex. Technologii proizvodstva i pererabotki produkzii zhivotnovodstva = Technology of production and processing of cattle breeding products, 2015, no. 1 (116), pp. 28–31.

6. Valitov F.R. Association of polymorphic variants of somatotropin and thyroglobulin genes with milk yields of Black-and-White cows. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta = Izvestia Orenburg State Agrarian University, 2018, no. 4 (72), pp. 284–287. (In Russian).

7. Pozovnikova M.V., Serdyuk G.N., Pogorelsky I.A., Tulinov O.V. Genetic structure of milk cows in relation to DNA-markers and influence of their genotypes on lactation performance. Molochnoye i miasnoye skotovodstvo = Dairy and Beef Cattle Farming, 2016, no. 2, pp. 8–12. (In Russian).

8. Safina N.Yu., Gilemkhanov I.Yu., Zinnatova F.F., Shakirov Sh.K. Characteristics of milk productivity of cows-heifers with different genotypes of somatotropin (GH). Vestnik Kazanskogo gosudarstvennogo agrarnogo universiteta = Vestnik of Kazan State Agrarian University, 2019, no. 3 (54), pp. 58–61. (In Russian). DOI: 10.12737/article_5db9535ed384a3.87060395.

9. Yulmet′eva Yu.R., Shakirov Sh.K. Involvement of lipid metabolism candidate genes in the productivity formation of cows. Molochnoye i miasnoye skotovodstvo = Dairy and Beef Cattle Farming, 2017, no. 1, pp. 10–13. (In Russian).

10. Sari E.M., Yunus M., Noor R.R., Sumantri C., Han J.L. Identification of single nucleotide polymorphism on growth hormone gene in Aceh cattle. Media peternakan, 2013, no. 1 (36), pp. 21–25. DOI: 10.5398/medpet.2013.36.1.21.

11. Özkan Ünal E., Sönmez G., Soysal M.İ., Kepenek E.Ş., Dinç H., Özer F., Togan İ.Z. Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle. Turkish journal of zoology, 2015, no. 5 (39), pp. 734– 748. DOI: 10.3906/zoo-1409-9.

12. Chessa S., Nicolazzi E.L., Nicoloso L., Negrini R., Marino R., Ajmone Marsan P., Vicario D., Valentini A. Analysis of candidate snps affecting milk and functional traits in the dual-purpose Italian Simmental cattle. Stefanon Livestock science, 2015, vol. 173, pp. 1–8. DOI: 10.1016/j.livsci.2014.12.015.