Preview

Siberian Herald of Agricultural Science

Advanced search
Open Access Open Access  Restricted Access Subscription Access

Epizootic situation and molecular genetic characteristics of the rabies virus isolated from the brain of domestic reindeer in Yakutia

https://doi.org/10.26898/0370-8799-2024-5-6

Abstract

Rabies was registered among horses, domestic reindeer and dogs on the territory of the Republic of Sakha (Yakutia) in 2001– 2021. Rabies virus (Rabies lyssavirus) was detected in biomaterial from wild animals in Arctic foxes, red foxes, wolves, colts, ermine and sables. In the Arctic zone of Yakutia in 2023, rabies virus was isolated from a brain sample from one of the domestic reindeer of a herd showing clinical signs of severe agitation and aggression to humans. It is assumed that the reindeer was infected with rabies virus when bitten by a rabies-sick Arctic fox, as the range of distribution of these animals in the region is quite large. Rabies virus detection studies were conducted using polymerase chain reaction, enzyme immunoassay and fluorescent antibody method. The diagnosis of rabies was confirmed in all diagnostic tests. The nucleic acid fragments were isolated from the positive samples by the PCR method and their nucleotide sequences were determined. Analysis of the nucleotide sequences of the viral genome revealed phylogenetic relatedness of the isolated rabies virus isolate in the Arctic zone of Yakutia with the rabies viruses circulating in some areas of the North American continent, including Greenland (two isolates), Canada (six), and the United States (three). The isolate of the rabies virus from the brain of domestic reindeer in the Arctic zone of Yakutia belongs to the group of strains of American origin (USA1) and is phylogenetically close to the strains isolated in Greenland, Canada, and the USA. The studied virus isolate territorially corresponds to the known antigenic variant 3, established earlier in the north of the Asian part of Russia, in particular in Yakutia.

About the Authors

P. L. Petrov
Department of Veterinary Medicine of the Republic of Sakha (Yakutia)
Russian Federation

Petr L. Petrov, Supervisor

Yakutsk



Yu. I. Smolyaninov
Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences. Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation

Yury I. Smolyaninov, Doctor of Science in Veterinary Medicine, Professor, Head Researcher

PO Box 463, Krasnoobsk, Novosibirsk Region, 630501



G. P. Protodiakonova
Arctic State Agrotechnological University
Russian Federation

Galina P. Protodiakonova, Doctor of Science in Veterinary Medicine, Associate Professor, Department Head

Yakutsk



M. A. Stepanyuk
State Scientific Center of Virology and Biotechnology "Vector" of the Rospotrebnadzor
Russian Federation

Marina A. Stepanyuk, Post-graduate Student

Koltsovo, Novosibirsk Region



V. A. Ternovoy
State Scientific Center of Virology and Biotechnology "Vector" of the Rospotrebnadzor
Russian Federation

Vladimir A. Ternovoy, Candidate of Science in
Biology, Department Head

Koltsovo, Novosibirsk Region



References

1. Gulyukin A.M., Smolyaninov Yu.I., Shabeikin A.A. The economic damage caused by rabies of agricultural animals in Russia. RJOAS = RJOAS, 2016, no. 8 (56), pp. 34–38.

2. Zaikova O.N., Grebennikova T.V., Elakov A.L., Kochergin-Nikitskii K.S., Aliper T.I., Gulyukin A.M., Chuchalin S.F. Molecular-genetic characterization of field isolates of rabies virus circulating on the territory of the Kirov region. Voprosy virusologii = Problems of virology, 2016, no. 4, pp. 186–192.

3. Zaikova O.N., Grebennikova T.V., Gulyukin A.M., Shabeikin A.A., Polyakova I.V., Metlin A.V. Molecular-genetic characterization of field isolates of rabies virus identified in the territory of Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions. Voprosy virusologii = Problems of virology, 2017, vol. 62, no. 3, pp. 101–108.

4. Gulyukin A.M., Shabeikin A.A., Makarov V.V., Zaikova O.N., Grebennikova T.V., Zaberezhnyi A.D., Polyakova I.V., Yuzhakov A.G. Features of epizootic process and molecular-genetic characteristics of virus isolates of rabies in the Tver region. Voprosy virusologii = Problems of virology, 2018, vol. 63, no. 3, pp. 115–123.

5. Yakovchits N.V., Bondarenko A.N., Adel'shin R.V., Noskov A.K., Andaev E.N., Botvinkin A.D. Rabies virus in the Krasnoyarsk Territory: two independent phylogroups. Dal'nevostochnyi zhurnal infektsionnoi patologii = The Far Eastern Journal of Infectious Pathology, 2019, no. 37, pp. 38–39.

6. Polishchuk E.M., Sidorov G.N., Nashatyreva D.N. Rabies in the Russian Federation. Omsk: Publishing Center, 2019, 110 p.

7. Chupin S.A., Sprygin A.V., Zinyakov N.G., Guseva N.A., Shcherbinin S.V., Korennoy F.I., Adelshin R.V., Mazloum A., Sukharkov A.Y., Nevzorova V.V. Phylogenetic Characterization of Rabies Virus Field Isolates Collected from Animals in European Russian Regions in 2009–2022. Microorganisms, 2023, vol. 11, p. 2526. DOI: 10.3390/microorganisms11102526.

8. Yakovchits N.V., Adelshin R.V., Zarva I.D., Chupin S.A., Melnikova O.V., Andaev E.I., Shulpin M.I., Metlin A.E., Botvinkin A.D. Fox rabies outbreaks in the republic of Buryatia: Connections with neighbouring areas of Russia, Mongolia and China. Transboundary and Emerging Diseases, 2021, vol. 68, is. 2, pp. 427–434. DOI: 10.1111/tbed.13692.

9. Ye Feng, Yuyang Wang, Hada, Deijide, Gaosuyilatu, Xin Li, Zemin Xu, Hasibagen, Amur Bulage, Linchuan Li, Sarula Yu Guo, Jihong Ma, Zhanying Kou, Sheng Sun, Liang Zhang, Tingfang Liu, Weidi Xu, Huachao Feng, Zihan Zhao, Zhongzhong Tu, Yan Liu, Changchun Tu. Diversity of rabies virus detected in Inner Mongolia, China, 2019–2021. Transboundary and Emerging Diseases, 2022, vol. 69, is. 2, pp. 249–253.

10. Kumar S., Stecher G., Kumar K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 2016, no. 33, pp. 1870–1874.


Review

For citations:


Petrov P.L., Smolyaninov Yu.I., Protodiakonova G.P., Stepanyuk M.A., Ternovoy V.A. Epizootic situation and molecular genetic characteristics of the rabies virus isolated from the brain of domestic reindeer in Yakutia. Siberian Herald of Agricultural Science. 2024;54(5):64-71. (In Russ.) https://doi.org/10.26898/0370-8799-2024-5-6

Views: 123


ISSN 0370-8799 (Print)
ISSN 2658-462X (Online)