Monitoring of coagulase-negative staphylococci isolated from the bulk milk in the farms troubled by cow mastitis

   One of the important aspects that guarantee product safety is constant sanitary and microbiological control at all stages of production. This study presents an analysis of monitoring investigations aimed at determining the spectrum of common species of coagulase-negative staphylococci in the bulk milk at four mastitis-unfavorable dairy farms in the Moscow region during one year. It was found that Staphylococcus epidermidis was detected more frequently than other species (42.9 % of the number of the isolated coagulase-negative staphylocococci), St. saprophyticus and St. simulans were detected less frequently (14.3 % each), St. haemolyticus, St. chromogenes, St. lentus and St. hominis were registered even less frequently (7.1 % each). It was determined that up to 28.0 % of bacteria of the genus Staphylococcus were isolated in the bulk milk in autumn on all the farms. The ratio of St. aureus and coagulase-negative staphylococci in the bulk milk on the farms of the Moscow region in the autumn period is presented. Identification of coagulase-negative staphylococci isolated during the autumn lactation period has been performed. In the bulk milk, St. saprophyticus and St. epidermidis were detected more frequently (23.1 % each), St. simulans and St. chromogenes were detected in 15.4 % of the cases, and St. hominis, St. haemolyticus, and St. lentus were detected somewhat less frequently (7.7% each). Average data on Staphylococcus aureus indication obtained for 2023 in different farms of the Moscow region from the bulk milk have been analyzed. Staphylococci levels in the bulk milk were highest in spring (38.1 %), summer and autumn data were approximately the same (25.3–28.0 %). The lowest level of staphylococci in the bulk milk was observed in winter (18.5 %).

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