Development of a device for diagnostics of garden strawberry spot diseases

   Symptoms of garden strawberry leaf spot disease caused by phytopathogenic fungi are described. The impossibility of their identification by visible signs in the initial stage of the lesion is shown. Early diagnosis is possible using antibody-based methods with phytopathogen antigens, molecular assays and aptamer-based methods. However, there are a number of difficulties in using them. To create a portable means of early diagnosis, methods based on the detection of electrical changes in plant leaf tissues as a result of fungal pathogen attack have been proposed: the method of electrical impedance spectroscopy and the method of electrical equivalent circuits. The methods allow determining a diagnostic parameter, the condenser capacitance modeling the capacitance of leaf plant cell membranes. It has been found that condenser capacitance values decrease as the degree of strawberry leaf lesion increases. The proposed methods are realized in a device consisting of an electrode system, electronic and computing units. In the electrode system, non-polarizing electrodes are located on both sides of the garden strawberry leaf. The electronic unit is designed to record the plant tissue impedance. The computing unit (laptop) searches for the frequency at which the reactive component of the impedance is maximum and calculates the diagnostic parameter from these data. The device operates under the control of a program for recording the reactive component of plant tissue impedance and a program for determining the diagnostic parameter. To evaluate the accuracy of the device, the values of the diagnostic parameter obtained by the developed device and the precision impedance analyzer WK 6505B were compared. The values of the parameter were 1594 and 1574 pF, respectively.

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