Toxicological assessment of the veterinary drug Trametin Plus

One of the complex problems facing veterinary science and practice is the control of associated respiratory and gastrointestinal diseases of young cattle. Improvement of the system of measures to reduce morbidity involves, among other things, the development and use of new therapeutic and prophylactic drugs. Trametin Plus, created on the basis of a modified biologically active substance obtained by liquid-phase fermentation of the xylotroph fungus Trametes pubescens (Shumach. Fr.) Pilat (strain 0663), can be used as such a preparation. The development of new drugs requires careful toxicological and biological substantiation of safety of their use both in veterinary medicine and in medicine according to the criteria of general toxic effect, remote and specific consequences. As a result of the conducted studies, it was revealed that according to the parameter of the average lethal dose (DL50) the tested drug at intragastric route of intake is characterized as a substance with low danger of acute toxicity and belongs to the V class of danger (DL50 > 2000 mg/kg). The magnitudes of the average lethal doses indicate that there are no differences in the sex sensitivity of the animals to this drug. During the study of cumulative properties in the subchronic toxicity test, no cases of animal death and signs of intoxication in white rats were observed, the cumulation coefficient could not be determined, which allows us to attribute the investigated veterinary drug to the group of substances that do not have cumulative properties. When evaluating the sensitizing properties of the drug by intradermal sensitization of guinea pigs, it was shown that Trametin Plus has weakly expressed allergenic properties. The study concluded that the studied drug is non-toxic and safe and can be used for further veterinary studies on farm animals. 

1. Chkhenkeli V.A., Chkhenkeli G.D., Nikonova A.A., Gorshkov A.G. Determining the fatty acids, polar and non-polar volatile organic compounds of the veterinary preparation "trametin plus". Izvestiya vuzov. Prikladnaya khimiya i biotekhnologiya = Proceedings of Universities. Applied Chemistry and Biotechnology, 2023, no. 13 (3), pp. 382–391. (In Russian). DOI: 10.21285/2227-2925-2023-13-3-382-391.

2. Arisova G.B. Toxicological characteristics of a veterinary antiparasitic preparation with a prolonged action in the form of a solution for internal admission. Teoriya i praktika bor’by s parazitarnymi boleznyami = Theory and practice of parasitic disease control, 2021, is. 22, pp. 67–73. (In Russian). DOI: 10.31016/978-5-6046256-13.

3. Stepanov L.F., Arisov M.V., Frisova G.B. Toxicity Assessment of a multicomponent antiparasitic drug in animals. Words veterinary journal, 2020, no. 10 (2), pp. 207–215. DOI: 10.36380/scil.2020.wvj27.

4. Indyukhova E.N., Arisov M.V., Arisova G.B., Stepanova I.A. Toxicological evaluation of the complex insectoacaricidal preparation "Neoterika Protecto 12". Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology, 2018, no. 12 (3), pp. 60–66. (In Russian). DOI: 10.31016/1998-8435-2018-12-3-60-66.

5. Rakhmatullin E.K., Sklyarov O.D Preclinical study of the veterinary drugs toxicity. Vestnik rossiyskoy sel’skokhozyaistvennoy nauki = Vestnik of the Russian agricultural science, 2019, no. 5, pp. 61–64. (In Russian). DOI: 10.30850/vrsn/2019/5/61-64.

6. Bazhenova E.Y., Fursenko D.V., Khotskin N.V., Sorokin I.E., Kulikov A.V. Effect of lethal yellow (AY) mutation and photoperiod alterations on mouse behavior. Vavilov journal of genetics breeding, 2019, vol. 23, no. 1, pp. 55–61. DOI: 10.18699/VJ19.461.

7. Belyakov V.I., Gromova D.S., Popova N.R., Myakisheva Yu.V. Modern methods for studying rodent behaviour in model biomedical studies (problem review). Sovremennye voprosy biomeditsiny = Modern Issues of Biomedicine, 2022, vol. 6, no. 4, pp. 13–22. (In Russian). DOI: 10.51871/2588-0500-2022-06-04-1.

8. Lugovik I.A., Makarova M.N. Toxicological studies. Reference intervals of mass coefficients of internal organs in a sample of 1000 rats. Laboratornye zhyvotnye dlya nauchnykh issledovaniy = Laboratory Animals for Science, 2021, no. 1, pp. 3–11. (In Russian). DOI: 10.29296/2618723X-2021-01-01.

9. El’kov A.N., Goldfarb Yu.S., Potskhveria M.M., Badalyan A.V. Optimization of statistical processing of clinical and laboratory data in acute poisoning. Toksikologicheskiy vestnik = Toxicological Review, 2021, no. 2, pp. 14–22. (In Russian). DOI: 10.36946/0869-7922-2021-2-14-22.

10. Yakimovich I.Yu., Gusakova S.V., Kotlovsky M.Yu., Podrezov I.K., Ivanov V.V., Vasiliev V.N., Portnova N.A., Prokopyeva A.V., Churin A.A., Dygai A.M. Behavioral reactions of white rats in the test "open field" under influence of regular physical load of different character. Krymskiy zhurnal eksperimental’noy i klinicheskoy meditsiny = Crimean journal of experimental and clinical medicine, 2019, vol. 9, no. 3, pp. 48–51. (In Russian).

11. Kirsanova A.Yu., Kubrak N.V. Quantitative estimation of urine sediment in healthy Wistar rats. Laboratornye zhyvotnye dlya nauchnykh issledovaniy = Laboratory Animals for Science, 2022, no. 1, pp. 3–7. (In Russian). DOI: 10.29296/2618723X-2022-01-01.

12. Yushkov B.G., Korneva E.A., Chereshnev V.A. The concept of norm in physiology and pathology. Physiological constants of laboratory animals. Ekaterinburg, 2021, 864 p. (In Russian).

13. Voloshan O.A., Gorshkov D.A., Petrova O.V., Ivanov P.A., Nikulina D.M. Determination of laboratory rats blood parameters with the formation of a regional protocol for experimental studies. Astrakhanskiy meditsinskiy zhurnal = Astrakhan Medical Journal, 2023, vol. 18, no. 2, pp. 47–54. (In Russian). DOI: 10.29039/1992- 6499-2023-2-47-54.

14. Stekolnikov A.A., Reshetnyak V.V., Burdeyniy V.V., Iskaliev E.A. Biochemical parameters of blood of mongrel rats. Mezhdunarodniy vestnik veterinarii = International Journal of Veterinary Medicine, 2020, no. 3, pp. 163–168. (In Russian). DOI: 10.17238/issn2072-2419.2020.3.163.

15. Voytenko N.G., Makarova M.N., Zueva A.A. Variability of blood biochemical parameters and establishing of reference ranges in nonclinical studies. Part 1: rats. Laboratornye zhyvotnye dlya nauchnykh issledovaniy = Laboratory Animals for Science, 2020, no. 1, pp. 47–53. (In Russian). DOI: 10.29296/2618723X-2020-02-01.