Interbreeding differences in hematologic parameters of cows in Western Siberia

The paper presents the results of the analysis of the content and variability of hematological status indicators in cows of different breeds bred in Western Siberia. Data on the assessment of differences in the absolute number of leukocytes, erythrocytes, platelets, hemoglobin concentration, relative number of leukocyte cells, stress and adaptation indices between animals of Holstein, Black-and-White, Red Steppe breeds are demonstrated. The peripheral blood of healthy cows of the 2nd lactation was examined. Hematological parameters were measured using the PCT 90Vet automatic hematology analyzer. The leukocyte formula was calculated in blood smears fixed in 950 ethanol and stained according to Romanovsky. Adaptation and stress indices were calculated. Robust statistical estimation and descriptive statistics methods were used. The Kruskal-Wallis criterion was used to compare the groups. Post hoc comparisons were carried out using the Dunn’s method. In most cases, the data distribution was different from normal. The average values of the estimated hemogram parameters in all cows varied within the physiological norm generally accepted for cattle. Holstein cows were characterized by the highest adaptive potential: the number of leukocytes was 12.1 × 109 /l, the relative number ofsegmented neutrophils was 37.5%, the adaptation index was 3.17, the stress index was 0.29. Interbreed differences were revealed in all evaluated indicators with the exception of band neutrophils (p < 0.05). The greatest influence of the breed factor has been established for eosinophils, erythrocytes and platelets; the lowest for monocytes. The value of n2 for these indicators was 0.43; 0.29; 0.29; 0.44, respectively. The data obtained reflect the differentiated contribution of genetic factors to different hematological parameters.

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