Development of the methods for growing healthy potato microplants of the Solnechny variety

The effect of different growing conditions, including different compositions of nutrient medium and different sources of illumination, on the development of potato microplants of the Solnechny variety under in vitro laboratory conditions was studied. Using full-spectrum LED lamps and modified Murashige-Skoog nutrient medium with reduced agar-agar content, plants showed a 25% increase in height and a 19% decrease in lamina surface area. The microplants grown using full-spectrum LED lamps and modified Murashige-Skoog nutrient medium with reduced agar-agar content and addition of indolylacetic acid at a concentration of 0.1 mg/ l differed from the control ones by reduced height (by 23%), the number of internodes (by 24%) and the number of leaves (by 26%). Using full-spectrum LED lamps and modified Murashige-Skoog nutrient medium with reduced agar-agar content (4 g/l) and addition of indolylacetic acid at a concentration of 0.5 mg/l, a decrease in the number of internodes by 24%, leaves by 14, lamina surface area by 39%, acceleration of rhizogenesis and increase in root system weight by 117% were observed. Microplants grown using linear LED lamps and modified Murashige-Skoog nutrient medium supplemented with indolylacetic acid at a concentration of 0.1 mg/l were 70% taller than controls, had 48% reduced leaf mass, 53% reduced lamina surface area, and 36% increased stem mass. Microplants grown using linear LED lamps and modified Murashige-Skoog nutrient medium showed a 73% increase in stem height, a 20% or 1 unit increase in the number of internodes, a 17% increase in root system length, an acceleration of rhizogenesis, and a 39% decrease in leaf area. Methods of cultivation of healthy potato microplants of the Solnechny variety have been developed, aimed at accelerated generation of a large number of plants and preparation of microplants for transplanting to aerohydroponic plants.

1. Oves E.V., Gaitova N.A., Edtzaeva K.T., Stoyanova E.M. Modern methods of releasing potato varieties from viruses (review). Zemledelie = Zemledelie, 2024, no. 5, рр. 37–42. (In Russian).

2. Barsukova E.N., Chibizova A.S. Effect of spectrum of the light-emitting diode lightning upon the process of micropropagation of the virus-free potato plants of different varieties. Agrarnyi vestnik Primor'ya = Agrarian Bulletin of Primorye, 2019, no. 1 (13), рр. 18–22. (In Russian).

3. Mazaeva Yu.V. Features of obtaining original seed potatoes using biotechnology methods. Nauka i obrazovanie = Science and education, 2023, no. 2, p. 176. (In Russian).

4. Smirnova Yu.D., Podolyan Yu.A. Techniques for increasing the efficiency of microclonal propagation of potatoes (review). Agrarnaya nauka Evro-Severo-Vostoka = Agricultural Science Euro-North-East, 2024, no. 3, рр. 319–329. (In Russian). DOI: 10.30766/2072-9081.2024.25.3.319329.

5. Bakunov A.L., Rubtsov S.L., Milekhin A.V., Dmitrieva N.N. Potato regenerants morphogenesis intensity when using lighting of various spectral composition. Vestnik KrasGAU = Bulletin KrasSAU, 2024, no. 7 (208), рр. 3–12. (In Russian).

6. Vlasevskaya E.A., Mukhametshin I.G. Influence of the nutrient medium and photoperiod on tuberization of potato micro-plants of promising potato varieties in in vitro cultire. Byulleten' nauki i praktiki = Bulletin of Science and Practice, 2019, no. 12, рр. 177–181. (In Russian).

7. Papikhin R.V., Pugacheva G.M., Muratova S.A., Mazaeva Yu.V., Nikonov K.E. Factors affecting potato microtubing. Nauka i obrazovanie = Science and education, 2021, no. 1. (In Russian).

8. Anikina I.N., Kusainov A.A., Zhagiparova M.E., Insebaeva M.K. The temperature factor of cultivation as a regulator of potato plant morphogenesis in vitro. Vestnik Evraziiskogo natsional'nogo universiteta imeni L.N.Gumileva. Seriya: Biologicheskie nauki = Bulletin of the L.N. Gumilyov Eurasian National University Bioscience Series, 2023, no. 4 (145), рр. 54–62. (In Russian).

9. Oves E.V., Gaitova N.A. The use of microplant thermotherapy to free potato varieties from viruses. Vestnik Torajgyrov universiteta. Khimiko-biologicheskaya seriya = Bulletin of Toraighyrov University. Chemistry & Biology series, 2021, no. 4, рр. 96–109. (In Russian).

10. Shanina E.P., Ust'yantsev A.V., Oberyukhtin D.A., Likhodeevskii G.A. The influence of the spectral composition of light from LED lamps on the development of potato plants in vitro. Dostizheniya nauki i tekhniki APK = Achievements of Science and Technology of the AIC, 2023, no. 10, рр. 34–40. (In Russian). DOI: 10.53859/02352451_2023_37_10_34.

11. Lisina T.N., Burdysheva O.V., Sholgin E.S., Latypova A.L., Eliseeva A.D. Research of the effect of light spectral composition on potato plants in vitro of Nevsky, Kamensky, Udacha varieties. Permskii agrarnyi vestnik = Perm Agrarian Journal, 2023, no. 2 (42), рр. 34–42. (In Russian). DOI: 10.47737/2307-2873_2023_42_34.

12. Varushkina A.M., Yakhina A.I., Shirinkina A.S., Tsema L.G., Latypova A.L. The influence of lighting spectral composition on physiological response of potato in vitro. Agrarnyi nauchnyi zhurnal = Agrarian Scientific Journal, 2021, no. 4, рр. 8–11. (In Russian). DOI: 10.28983/asj. y2021i4pp8-11.

13. Basiev S.S., Gazdarov M.D., Tamakhina A.Ya., Gazzaev G.T., Abaev A.A. Impact of lighting quality and the nutrient medium composition on the growth and development of potato plants in vitro. Izvestiya Gorskogo gosudarstvennogo agrarnogo universiteta = News of the Gorsky State Agrarian University, 2022, vol. 59-4, рр. 18–25. (In Russian). DOI:10.54258/20701047_2022_59_4_18. 14.

14. Mazaeva Yu.V., Pugacheva G.M. Influence of different nutrient media on the efficiency of growth and development of potato plants in vitro and tuberization. Nauka i obrazovanie = Science and education, 2021, no. 2. (In Russian).

15. Romanova M.S., Khaksar E.V., Novikov O.O., Leonova N.I., Semenov A.G. The effect of different compositions of growth media on the development of microplants of the Antonina potato variety. Sibirskii vestnik sel'skokhozyaistvennoi nauki = Siberian Herald of Agricultural Science, 2020, no. 6, рр. 26–36. (In Russian). DOI: 10.26898/0370-8799-2020-6-3.