Study of the biochemical composition of sturgeon blood using a cyclodextrin complex with enterosgel

The biochemical picture of blood allows not only to determine the effect of antimicrobial drugs on the body of fish, adaptation to the conditions of water bodies, but also to determine the physiological state of aquatic organisms. The purpose of the study was to study the effect of cyclodextrin complex with enterosgel on the biochemical parameters of the blood of young sturgeons. For the studies, experimental groups were formed according to the principle of pairs-analogues in the amount of 10 specimens in each group: 1st control and 2nd control groups were characterized by an impaired functional state of the body. No therapeutic measures were carried out with them, levofloxacin with enterosgel was not introduced into the compound feed. The differences were that individuals of the 1st control group were given high-quality feed, while the 2nd control group was given poor-quality feed. The 1st experimental and 2nd experimental groups received high-quality feed, they underwent therapeutic measures, and they were characterized by an impaired functional state of the body. The differences consisted in different concentrations of the administered drug – 15 and 10%, respectively. The total protein content of fish on the 8th day in the 1st and 2nd experimental groups was 77.60 ± 0.80 and 84.90 ± 3.43 g / l, respectively, which is 7.87 g / l and 0.57 g / l lower than in the 1st control group. In terms of total protein content, the control groups had virtually no differences between themselves with 86.33 g / l and 86.63 g / l respectively. In sturgeons from the experimental groups, the indicator was lower than the control values by 9.76 g / l and 3.86 g / l. Analysis of the content of macro elements showed that their values by groups were approximately at the same level and did not go beyond the norm. According to the obtained values of phosphorus concentration in the blood, the optimal values were observed in sturgeons of the 2nd experimental group, which received the studied complex with a fluoroquinolone content of 10%.

1. Garlov P.E., Arshanitsa N.M., Stekolnikov A.A., Grebtsov M.R., Bugrimov B.S. The possibility to recreate sturgeon populations in the NorthWest. Mezhdunarodny`j vestnik veterinarii = International Journal of Veterinary Medicine, 2020, no. 4, pp. 116–123. (In Russian). DOI: 10.17238/issn2072-2419.2020.4.116.

2. Zhandalgarova A.D., Bakhareva A.A., Grozesku Yu.N., Nevalennyy A.N. Study of microbiocenosis of fish gills and intestines in their cultivation using preparations based on bacteriophages. Vestnik Astrahanskogo gosudarstvennogo tekhnicheskogo universiteta. Seriya: Rybnoe hozyajstvo = Vestnik of Astrakhan State Technical University. Series: Fishing Industry, 2021, no. 4, pp. 126–133. (In Russian). DOI: 10.24143/2073-5529-2021-4-126-133.

3. Ahmedzhanova A.B., Ponomarev S.V., Fedorovykh Yu.V., Levina O.A., Terganova N.V., Hisamutdinova A.N. Study of the effectiveness of using the antioxidant astaxanth in the composition of feeds for valuable aquaculture objects. Rybovodstvo i rybnoe hozyajstvo = Fish Breeding and Fisheries, 2023, vol. 17, no. 1 (204), pp. 42–52. (In Russian). DOI: 10.33920/sel-09-2301-04.

4. Vlasenko L.V., Kosyan D.B. Metal nanoparticles as potential inhibitors of «Quorum sensing» systems in bacteria (review). Zhivotnovodstvo i kormoproizvodstvo = Animal Husbandry and Fodder Production, 2021, vol. 104, no. 2, pp. 8–15. (In Russian). DOI: 10.33284/2658-3135-104-2-8.

5. Grachev M.K., Terekhova I.V., Shipilov D.A. Dimeric (oligomeric) derivatives of cyclodextrins as a new class of supramolecular systems. Synthesis and inclusion complexes on their basis. Bioorganicheskaya himiya = Russian Journal of Bioorganic Chemistry, 2020, vol. 46, no. 1, pp. 18–35. (In Russian). DOI: 10.31857/S0132342320010029.

6. Poddubnaya I.V., Rudneva O.N., Gurkina O.A. Changes in slaughter characteristics of sturgeon under the application of polymer systems with levofloxacin. Vestnik Ul`yanovskoj gosudarstvennoj sel`skoxozyajstvennoj akademii = Vestnik of Ulyanovsk State Agricultural Academy, 2024, no. 4 (68), pp. 151–158. (In Russian). DOI: 10.18286/1816-4501-2024-4-151-158.

7. Le-Deygen I.M., Skuredina A.A., Kudryashova E.V. Experimental methods for studying the mechanisms of interaction of lipid membranes with low-molecular-weight drugs. Bioorganicheskaya himiya = Russian Journal of Bioorganic Chemistry, 2020, vol. 46, no. 4, pp. 340–359. (In Russian). DOI: 10.31857/S013234232004017X.

8. Sutyagin A.A. Desilylation of β-cyclodextrin derivative modified with phosphocyclic fragments. Izvestiya vy`sshix uchebny`x zavedenij. Seriya Ximiya i ximicheskaya texnologiya = Chem-ChemTech, 2024, vol. 67, no. 11, pp. 33–39. (In Russian). DOI: 10.6060/ivkkt.20246711.7054.

9. Skuredina A.A., Kornova T.Ya., Le-Deygen I.M., Kudryashova E.V. Physico-chemical properties of guest-host inclusion complexes of ciprofloxcin with β-cyclodextrin derivatives. Vestnik Moskovskogo universiteta. Seriya 2. Himiya = Bulletin of Moscow University. Series 2. Chemistry, 2020, vol. 61, no. 4, pp. 278–286. (In Russian). DOI: 10.31857/S0132342320050243.

10. Kutyasheva N.V., Kurochkina G.I., Glushko V.V., Grachev M.K. Features of formation of inclusion complexes of mono-substituted β-cyclodextrin derivatives. Zhurnal obshhej ximii = Russian Journal of General Chemistry, 2021, vol. 91, no. 6, pp. 959–965. (In Russian). DOI: 10.31857/S0044460X21060135.

11. Halavach T.N., Lovkis Z.V., Kurchenko V.P., Yantsevich A.V., Tarun E.I., Dudchik N.V., Rzhepakovsky I.V., Lodygin A.D. Biologically active dairy protein hydrolysates and their cyclodextrin inclusion complexes: biotechnological aspects of production. Tekhnika i tekhnologiya pishchevyh proizvodstv = Food Processing: Techniques and Technology, 2024, vol. 54, no. 3, pp. 461–482. (In Russian). DOI: 10.21603/2074-9414-2024-3-2521.

12. Kilyakova Yu.V., Miroshnikova E.P., Arinzhanov A.E., Saldeeva K.A. Biological effect of feed probiotic additives «Subtilis-Zh» and «Atysh» on the body of carp yearlings. Part 1. Blood counts. Rybovodstvo i rybnoe hozyajstvo = Fish Breeding and Fisheries, 2022, vol. 16, no. 10 (201), pp. 701–711. (In Russian). DOI: 10.33920/sel-09-2210-05.

13. Kilyakova Yu.V., Miroshnikova E.P., Arinzhanov A.E., Mingazova M.S. Use of feed additives with sorption properties in fish farming: literature review. Veterinarnaya patologiya = Russian Journal of Veterinary Pathology, 2024, vol. 23, no. 4, pp. 52–66. (In Russian). DOI: 10.23947/2949-4826-2024-23-4-52-66.

14. Korotkiy V.P., Maxim E.A., Yurin D.A., Yozhkin M.A., Ryzhov V.A. Influence of new feed additives into the sterlet diets on its productivity. Zootexniya = Zootechniya, 2024, no. 4, pp. 32–35. (In Russian). DOI: 10.25708/ZT.2024.73.51.009.

15. Kochetkov N.I., Nikiforov-Nikishin D.L., Smorodinskaya S.V., Klimuk A.A., Golovacheva N.A. Effects of dietary lactobacillus brevis 47f on growth performance, hematological and histological parameters of juvenile sterlet (Acipenser Ruthenus). Ry`bnoe xozyajstvo = Fisheries, 2024, no. 4, pp. 96–107. (In Russian). DOI: 10.36038/0131-6184-2024-4-96-107.