Biological properties of saprophytic fungi species common in the Republic of Kazakhstan (using the Alternaria alternata isolate as an example)

In the last decade, many researchers have noted a significant increase in various pathologies in domestic and farm animals caused by saprophytic fungal species. Clinically, they manifest themselves as local lesions of the skin and coat, or are accompanied by systemic lesions of the internal organs of animals. Fungi of the genus Alternaria are most often associated with plant pathologies, although cases of animal diseases caused by the representatives of this genus have been described in the scientific literature. The purpose of the work was to isolate the fungus A. alternata on special nutrient media, to study its cultural, morphological and biological properties. For this purpose, samples of biological material were taken from a horse with clinical signs of skin damage. Molecular genetic studies, species identification and determination of the biological properties of the isolated culture were carried out using approved methodological recommendations and determinants of pathogenic and conditionally pathogenic fungi. The cultural, morphological (phenotypic), keratinolytic, biochemical, and molecular genetic properties of an opportunistic species of the microscopic fungus A. alternata have been studied. The isolate was assigned the number 15.23.7.1H; it formed a greenish-brown airy and various shades of black substrate mycelium. Its microstructures were represented by coffee-brown mycelium and conidia. Biochemical properties were characterized by the ability to absorb mannitol and urea, poor absorption of glucose, and the inability to break down sucrose, maltose, lactose, casein, gelatin, and peptone. It had pronounced β-hemolytic and keratinolytic activity. Molecular genetic studies have established the identity of the nucleotide sequences of isolate 15.23.7.1H of A. alternata with the strain sequences published in GenBank. Its nucleotide sequences are deposited in the GenBank database under the number PV793443.1.

1. Cafarchia C., Figueredo L.A., Otranto D. Fungal diseases of horses // Veterinary Microbiology. 2013. Vol. 167. P. 215–234. DOI: 10.1016/j.vetmic.2013.01.015.

2. Fisher M.C., Henk D.A., Briggs C.J., Brownstein J.S., Madoff L.C., Mc Craw S.L., Gurr S.J. Emerging fungal threats to animal, plant and ecosystem health // Nature. 2012. Vol. 484. P. 186–194.

3. Brás S., Sabino R., Laureano A., Simões H., Fernandes C., Marques-Pinto G., Cardoso J., Veríssimo C. Cutaneous infection by different Alternaria species in a liver transplant recipient // Medical Mycology Case Reports. 2015. Vol. 8. P. 1–4. DOI: 10.1016/j.mmcr.2015.01.004.

4. Nichita I., Marcu A. The fungal microbiota isolated from cats and dogs // Scientific Papers: Animal Science and Biotechnologies. 2010. Vol. 43. P. 411–414.

5. Paixao G.C., Sidrim J.J.C., Campos G.M.M., Brilhante R.S.N., Rocha M.F.G. Dermatophytes and saprobe fungi isolated from dogs and cats in the city of Fortaleza, Brazil // Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 2001. Vol. 53. P. 568–573. DOI: 10.1590/S010209352001000500010.

6. Soomra I.H., Shar A.H., Soomra F.M. Fungal biota of the domestic animals in a city in Pakistan // Pakistan Journal of Medical Sciences. 2010. Vol. 26. P. 964–967.

7. De Mers M. Alternaria alternata as endophyte and pathogen // Microbiology. 2022. Vol. 168 (3). P. 001153. DOI: 10.1099/mic.0.001153.

8. Turzhanova A., Khapilina O.N., Tumenbayeva A., Shevtsov V., Raiser O., Kalendar R. Genetic diversity of Alternaria species associated with black point in wheat grains // Peer Journal. 2020. Vol. 3. P. e9097. DOI: 10.7717/peerj.9097.

9. Fernandes C., Casadevall A., Gonçalves T. Mechanisms of Alternaria pathogenesis in animals and plants // FEMS Microbiology Reviews. 2023. Vol. 47 (6). P. fuad061. DOI: 10.1093/ femsre/fuad061.

10. Raza H., Khan R.U., Anwar K., Muhammad K. Visceral phaeohyphomycosis caused by Alternaria alternata offering a diagnostic as well as a therapeutic challenge // Saudi Journal of Kidney Diseases and Transplantation. 2015. Vol. 26 (2). P. 339–343. DOI: 10.4103/1319-2442.152503.

11. Mc Kay J.S., Cox C.L., Foster A.P. Cutaneous alternariosis in a cat // Journal Small Animals Practice. 2001. Vol. 42 (2). P. 75–78. DOI: 10.1111/j.1748-5827.2001.tb01996.x.

12. Dye C., Johnson E.M., Gruffydd-Jones T.J. Alternaria species infection in nine domestic cats // Journal Feline Medicine Surgery. 2009. Vol. 11 (4). P. 332–336. DOI: 10.1016/j. jfms.2008.07.005.

13. Dedola C., Stuart A.P., Ridyard A.E., Else R.W., van den Broek A.H., Choi J.S., de Hoog G.S., Thoday K.L. Cutaneous Alternaria infectoria infection in a dog in association with therapeutic immunosuppression for the management of immune-mediated haemolytic anaemia // Veterinary Dermatology. 2010. Vol. 21. P. 626–634. DOI: 10.1111/j.1365-3164.2009.00875.x.

14. Stojanov I.M., Jaksic S.M., Prodanov J.Z. Presence and importance of saprophyte fungal organisms on dog skin // Zbornik Matice srpske za prirodne nauke. 2007. Vol. 113. P. 261–265. DOI: 10.2298/ZMSPN0713261S.

15. Rajkumar S.S., Rajamanickam S.M. Opportunistic fungi as etiologic agents of dermatitis – a case of Alternaria fungal infestation in canines // International Journal of Livestock Research. 2015. Vol. 5. P. 24–28. DOI: 10.5455/ ijlr.20150421114244.

16. Dicken M., Munday J.S., Archer R.M., Mayhew I.G., Pandey S.K. Cutaneous fungal granulomas due to Alternaria spp. infection in a horse in New Zealand // New Zealand Veterinary Journal. 2010. Vol. 58 (6). P. 319–320. DOI: 10.1080/00480169.2010.69765.

17. Genovese L.M., Whitbread T.J., Campbell C.K. Cutaneous nodular phaeohyphomycosis in five horses associated with Alternaria alternata infection // Veterinary Record. 2001. Vol. 148 (2). P. 55–66. DOI: 10.1136/vr.148.2.55.

18. González-Medina S., Dukes J., Rasotto R., Szekely A., Borman A.M. Facial cutaneous phaeohyphomycosis associated with Alternaria infectoriae infection // Equine Veterinary Education. 2017. Vol. 31 (1). P. 13–18. DOI: 10.1111/ eve.12761.

19. Kukhar Y., Bailina G., Smagulova A., Uakhit R., Kiyan V. Characteristics of Chrysosporium spp. Pathogens Causing Skin Mycoses in Horses // Journal of Fungi. 2025. Vol. 11 (4). P. 297. DOI: 10.3390/jof11040297.

20. Seyedmousavi S., Guillot J., de Hoog G.S. Phaeohyphomycoses, emerging opportunistic diseases in animals // Clinical Microbiology Reviews. 2013. Vol. 26. P. 19–35.

21. Iyalla C. A review of the virulence factors of pathogenic fungi // Journal of Clinical and Experimental Microbiology. 2017. Vol. 18 (1). P. 53–58. DOI: 10.4314/ajcem.v18i1.8.

22. Kukhar E.V., Sharipova A.M., Shevtsov A.B. Selection of a method for DNA isolation from dermatomycetes and other micromycetes. Problemy meditsinskoy mikologii = Problems in medical mycology, 2013, vol. 15, no. 2, p. 95. (In Russian).

23. Touaitia R., Mairi A., Ibrahim N.A., Basher N.S., Idres T., Touati A. Staphylococcus aureus: a Review of the Pathogenesis and Virulence Mechanisms // Antibiotics. 2025. Vol. 14 (5). P. 470. DOI: 10.3390/antibiotics14050470.

24. Brunke S., Mogavero S., Kasper L., Hube B. Virulence factors in fungal pathogens of man // Current Opinion in Microbiology. 2016. Vol. 32. P. 89–95. DOI: 10.1016/j.mib.2016.05.010.

25. De Mers M. Alternaria alternata as endophyte and pathogen // Microbiology. 2022. Vol. 168 (3). P. 001153. DOI: 10.1099/mic.0.001153.

26. Lockhart S.R., Gary J.M. Is This Zebra Really a Zebra? The Challenge of Diagnosing Rare Fungal Infections in Veterinary Pathology // Veterinary Pathology. 2019. Vol. 56 (4). P. 510–511. DOI: 10.1177/0300985819843684.