GENETIC DIVERSITY OF CATTLE ON A COMPLEX OF GENOTYPES OF LOCI TNF-a AND TNFR1
Abstract
Genetic structure of certain cattle populations has been described on the basis of allele frequencies and various genotype combinations of polymorphic loci TNF-a (-824A/G, -793 C/T) and TNFR1. Herds of dairy cattle in Novosibirsk Region and Altai Territory were investigated. The animals of Red Steppe (n=148), Simmental (n=149) and Holstein (n=100) breeds were studied. The tumor necrosis factor alpha gene (TNF-a) encodes a multifunctional cytokine, one of the main functions of which is to be involved in the formation of the immune response. The cytokine biological effect on the cells is manifested through interaction with the specific receptor, encoded by the TNFR1 gene. The TNF-a cytokine and its TNFR1 receptor are part of the so-called tumor necrosis factor family. Proteins of the tumor necrosis factor family participate in metabolic and morphogenetic processes, and affect reproductive characteristics of farm animals. Genotypes were determined by the polymerase chain reaction method with subsequent analysis of the lengths of restriction fragments. Statistically significant interbreed differences on frequencies of alleles are set (p<0.01). Of the 27 possible combinations of genotypes for the three polymorphisms in animals, only 18 have been discovered. In Red Steppe breed were identified 16 different variants of genotypes, in Simmental and Holstein breeds 10 and 9, respectively. The most common genotypes were as follows: the G/A+T/C+T/C (19.9 percent) in Red Steppe breed, G/G+T/T+C/C (61.4 percent) in Simmental, a variant of the combination G/A+T/C+C/C (35.7 percent) in Holstein. There were significant differences between breed groups for the frequencies of occurrence of some genotypic intralocus and interlocus combinations (p<0.01– p<0.001).
About the Authors
T. I. Krytsyna
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Biotechnology Engineer,
160, Dobrolyubova St, Novosibirsk, 630039
N. N. Kochnev
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Doctor of Science in Biology, Professor,
160, Dobrolyubova St, Novosibirsk, 630039
N. S. Yudin
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Candidate of Science in Biology, Senior Researcher,
10, Akademika Lavrentyeva St, Novosibirsk, 630090
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Review
For citations:
Krytsyna T.I., Kochnev N.N., Yudin N.S. GENETIC DIVERSITY OF CATTLE ON A COMPLEX OF GENOTYPES OF LOCI TNF-a AND TNFR1. Siberian Herald of Agricultural Science. 2017;47(2):66-73. (In Russ.)
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