EFFICACY OF HYPERIMMUNE SERUM AGAINST SWINE ERYSIPELAS PRODUCED BY HYPERIMMUNIZED OXEN-PRODUCERS
Abstract
Materials on the spread of swine erysipelas in Krasnodar Territory are given, and the effectiveness of application of the immunomodulator Immunofarm to stimulate antibody production in the case of hyperimmunization of oxen-producers by the antigen of swine erysipelas was evaluated. From preliminary tests on white mice was established that immunomodulating potential of Immunofarm was most pronounced with three-fold usage before each administration of the antigen: quantity of antibodies in serum was 85.2% higher as compared to the control. The application of Immunofarm has proven to ensure the stable growth and maximum accumulation of specific antibodies to the causative agent of swine erysipelas in the blood of oxen-producers. A ten-fold cycle of hyperimmunization of oxen with increasing doses of the erysipelas antigen, in combination with Immunofarm according to the optimal scheme, ensures the stable growth and maximum accumulation of specific antibodies in blood serum of not lower than 167 EA/cm3 , which is 33.6% higher than in the control samples. When the fractional composition of industrial lots of hyperimmune serum was studied by the electrophoresis method in polyacrylamide gel with sodium dodecyl sulfate, the presence of light and heavy chains of IgG was established, which is a confirmation of the high level of immune reaction of the second type in oxen-producers. During the tests under production conditions, the high therapeutic efficacy of heterogeneous serum against swine erysipelas, obtained by means of application of Immunofarm, was established: the safety of pigs was 97%, which is not inferior to the therapeutic efficacy of homogeneous hyperimmune serum from pig-producers.
About the Authors
E. V. Susskiy
Armavir BioPlant
Russian Federation
Russian Federation
Doctor of Science in Biology, Director,
11, Mechnikova St, Progress, Novokubanskiy District, Krasnodar Territory, 352212
Yu. A. Glushenkova
Armavir BioPlant
Russian Federation
Russian Federation
Microbiologist,
11, Mechnikova St, Progress, Novokubanskiy District, Krasnodar Territory, 352212
Yu. E. Fedorov
Krasnodar Veterinary Research Institute
Russian Federation
Russian Federation
Candidate of Science in Veterinary Medicine, Researcher,
1,1st Line St, Krasnodar, 350004
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Review
For citations:
Susskiy E.V., Glushenkova Yu.A., Fedorov Yu.E. EFFICACY OF HYPERIMMUNE SERUM AGAINST SWINE ERYSIPELAS PRODUCED BY HYPERIMMUNIZED OXEN-PRODUCERS. Siberian Herald of Agricultural Science. 2017;47(3):55-61. (In Russ.)
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