PECULIARITIES OF THE STRUCTURE OF INTESTINAL VILLI IN MICE WITH METABOLIC SYNDROME
Abstract
The length of intestinal villi in the jejunum of mice with metabolic syndrome (MS) was studied. The aim of research was to study the state of the mucous membrane of the small intestine in mice with MS using the method of luminescence microscopy. The experiment was performed on C57/black 6 line mice, which were divided into 2 groups: control group animals received a feed with 5% fat content, experimental group animals a feed with 10% fat content. The mass of the internal organs was statistically significantly lower in the group fed with 5% fat feed than that in the group of animals with MS. Fragments of the jejunum were stained with Hoechst 33258 dye. The length of intestinal villi in the control group averaged 417 mcm. The length of the villi in the animal test group was on average 563 mcm. In mice with MS, the length of intestinal villi of the jejunal mucosa was 35% more than the analogous parameter in the control group mice (P < 0.05). The number of intestinal villi per unit area of the intestinal jejunum mucosa in mice with MS was lesser than that in mice of the control group (42 + 4 and 90 + 8 villi per 1 mm2 , respectively). The decrease in the number of intestinal villi per unit area of the jejunal mucosa in mice with MS is explained by the widening of the lumen of the small intestine. The increase in the suction area in mice with MS should be considered as a pathological cycle, which aggravates the development of MS.
About the Authors
A. S. Bobikova
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Student,
160, Dobrolyubova St, Novosibirsk, 630039
D. S. Boldyreva
Institute of Chemical Biology and Fundamental Medicine, SB RAS
Russian Federation
Russian Federation
Student,
8, Akademika Lavrentyeva St, Novosibirsk, 630090V. N. Afonyushkin
Institute of Chemical Biology and Fundamental Medicine, SB RAS;
Institute of Experimental Veterinary Science of Siberia and the Far East, SFSCA RAS
Russian Federation
Russian Federation
Candidate of Science in Biology, Sector Head,
8, Akademika Lavrentyeva St, Novosibirsk, 630090;
Krasnoobsk, Novosibirsk Region, 630501
N. A. Sigareva
Novosibirsk State Agrarian University
Russian Federation
Russian Federation
Candidate of Science in Biology, Associate Professor,
160, Dobrolyubova St, Novosibirsk, 630039
References
1. Leshchenko D.V. Kostyuk N.V., Belyakova M.B., Egorova E.N., Minyaev M.V., Petrova M.B. Dieticheski indutsirovannye zhivotnye modeli metabolicheskogo sindroma (obzor literatury) // Verkhnevolzh. med. zhur. – 2015. – T. 14, vyp. 2. – S. 34–39.
2. Roytberg G.E. Metabolicheskiy sindrom / pod. red. G.E. Roytberga. – M.: MED-press-inform, 2007. – 224 s.
3. Body Weight Information for C57BL/6J (000664): URL: https://www.jax.org/jax-mice-and-services/straindata-sheet-pages/body-weight-chart-000664.
4. Gajda A.M. Pellizzon M.A., Ricci M.R., Ulman E.A. Diet Induced Metabolic Syndrome in Rodent Models animal // LABNEWS. – 2007. – March.
5. Miyoshi M. è et al. Anti-obesity effect of Lactobacillus gasseri SBT2055 accompanied by inhibition of pro-inflammatory gene expression in the visceral adipose tissue in diet-induced obese mice // Eur. J. Nutr. – 2014. – Ò. 53. – N 2. – Ñ. 599–606.
6. Clarke R.M. The effect of growth and of fasting on the number of villi and crypts in the small intestine of the albino rat // J. Anat. – 1972. – N 112. – P. 27–33.
7. Forrester J.M. The number of villi in rat’s jejunum and ileum: effect of normal growth, partial enterectomy, and tube feeding // J. Anat. – 1972. – Vol. 111. –P. 283–291.
8. Shulman D., Hu C., Duckett G. et al. Effects of short-term growth hormone therapy in rats undergoing 75% small intestinal resection //J. Pediatr. Gastroenterol Nutr, 1992. – N 14. – P. 3–11.
9. Drucker D.J. Biological actions and therapeutic potential of the glucagon-like peptides // Gastroenterology. – 2002. – N 122. – P. 531–544.
10. Xiao Q., Boushey R.P., Drucker D.J. et al. secretaryretaryretaryretaryretion of the intestinotropic hormone glucagon-like peptide 2 is differentially regulated by nutrients in humansGastroenterology. – 1999. – N 117. – P. 99–105
11. Jiaming Mao, Xiaomin Hu, Yao Xiao, Chao Yang, Yi Ding, Ning Hou, Jue Wang, Heping Cheng, Xiuqin Zhang. Overnutrition Stimulates Intestinal Epithelium Proliferation Through â-Catenin Signaling in Obese Mice // Diabetes. – 2013. – N 62 (11). – P. 3736–3746.
Review
For citations:
Bobikova A.S., Boldyreva D.S., Afonyushkin V.N., Sigareva N.A. PECULIARITIES OF THE STRUCTURE OF INTESTINAL VILLI IN MICE WITH METABOLIC SYNDROME. Siberian Herald of Agricultural Science. 2017;47(3):62-67. (In Russ.)
Views:
155
Email this article 