A STUDY ON THE EFFECT OF 0.1% SOLUTION OF EPINEPHRINE HYDROCHLORIDE ON RESISTANCE OF WHITE BLOOD CELLS IN LAMBS
Abstract
There was studied the effect of 0.1% solution of epinephrine hydrochloride on resistance of white blood cells in lambs. In 128 lambs at birth, the blood was sampled from the jugular vein to reproduce stress reactions of the peripheral blood system of newborns in vitro and in the Goryaev chamber. Resulted from evaluation of stress susceptibility, the lambs were divided into two groups. The experimental group was made up of the lambs with high susceptibility to 0.1% solution of epinephrine hydrochloride, the control one of the lambs with low susceptibility. By the number of intact and disrupted leukocytes to 0.1% solution of epinephrine hydrochloride into the peripheral blood system at 30, 60 minutes after incubation in a thermostat within animal body temperature, no significant differences between the control and experimental blood samples were found (p > 0.05). However, 120 minutes after incubation showed a significant difference (p < 0.05) by these blood parameters of the peripheral blood system. The newborn lambs with high stress susceptibility (experimental group) had the lesser number of intact leukocytes in the blood system than their contemporaries from the control group did. In the early postnatal period of growth and development the lambs from experimental and control groups differed in live weight and average daily liveweight gain. The stress-resistant lambs were superior to stress susceptible ones in health and production parameters. The method for determining stress susceptibility to epinephrine hydrochloride and a degree of its manifestation makes it possible to differentiate lambs from the early age for stress resistant and stress susceptible sheep offspring. This technique is necessary in what follows to develop the kernel of breeding stock and improve economic traits in animals.
About the Authors
G. M. Akhmadiyev
Kazan (Privolzhskiy) Federal University
Russian Federation
Russian Federation
Doctor of Science in Veterinary Medicine, Professor,
10 A, Syuyumbike Ave, Naberezhnye Chelny, 423812
N. N. Smirnova
Kazan (Privolzhskiy) Federal University
Russian Federation
Russian Federation
Candidate of Science in Biology, Associate Professor,
10 A, Syuyumbike Ave, Naberezhnye Chelny, 423812
R. N. Sharafutdinov
Kazan (Privolzhskiy) Federal University
Russian Federation
Russian Federation
Candidate of Science in Biology, Associate Professor,
10 A, Syuyumbike Ave, Naberezhnye Chelny, 423812
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For citations:
Akhmadiyev G.M., Smirnova N.N., Sharafutdinov R.N. A STUDY ON THE EFFECT OF 0.1% SOLUTION OF EPINEPHRINE HYDROCHLORIDE ON RESISTANCE OF WHITE BLOOD CELLS IN LAMBS. Siberian Herald of Agricultural Science. 2017;47(3):49-54. (In Russ.)
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