INFLUENCE OF AMLODIPINE ON RESISTANCE TO ENROFLOXACINE IN SALMONELLA ENTERICA

In recent years, there has been an increase in the number of strains of salmonellae resistant to antibiotics of fl uoroquinolone series. The occurrence of Salmonella resistant to enrofl oxacin was assessed at poultry farms of the Russian Federation in 2007 and 2017. In 2007 sensitivity of 23 salmonella cultures to enrofl oxacin was studied and in 2017 the same study was done with 15 salmonella cultures isolated from agricultural birds. Antibiotic resistance was determined by the disc-diffusion method in compliance with the generally accepted requirements. From 2007 to 2017 the frequency of salmonella isolation resistant to enrofl oxacin increased from 0 to 23.08%. Evaluation of the effl ux (active removal of antimicrobial substances by a bacterial cell) was carried out using a classical test with ethidium bromide. It was established that the presence of effl ux in Salmonella cultures was associated with an insignifi cant increase in resistance to enrofl oxacin, trimethoprim with sulfamethoxazole and trimethoprim with sulfadimezine. The effect of amlodipine on the sensitivity of salmonella to enrofl oxacin was evaluated by cultivation of Salmonella in the presence of various concentrations of amlodipine. It was revealed that the specifi c proportion of strains of salmonella with higher sensitivity to enrofl oxacin increased with the simultaneous rise in concentrations of amlodipine (Pearson correlation, r = 0.9). Administration of amlodipine in concentration of 250 μg/ml to Salmonella enterica cultures, in the presence of subinhibitory concentrations of enrofl oxacin, increased their sensitivity to enrofl oxacin in 87.5% of cases.

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