Hybridological analysis of gliadincoding loci of F2 wheat from interspecific hybrid combination
https://doi.org/10.26898/0370-8799-2019-1-12
Abstract
The paper presents the results of the study into splitting alleles of gliadincoding loci in F2 grains obtained from crossing specimens of Triticum durum Desf., v hordeiforme GR 8670 (2n = 4x = 28) and T. dicoccum v farum (2n = 4x = 28). The purpose of conducting interspecific hybridization was to study the alleles inheriting gliadincoding loci regulating synthesis of alleles storage proteins in wheat grains of hybrid generation F2 (T.durum v.hordeiforme GR 8670 x T.dicoccum v farum). To identify allelic blocks of gliadin components in the grains of F2 hybrids obtained, the earlier identified variety of the winter durum wheat Langdon was used. Alleles were identified in electrophoregram of gliadin storage proteins, in 96 grains of wheat of the hybrid generation F2 by polymorphism and frequency of occurrence of these alleles. The work confirmed the linked inheritance of electrophoretic components of gliadincoding loci according to the Mendel type of inheritance (1: 2: 1). The assessment of the reliability of splitting, according to the component composition ofgliadin, was performed by calculating the χ2 criterion. New allelic blocks of components in hybrid generation F2 of durum wheat varieties v.hordeiforme GR 8670 Gli A1def and Gli B1dfb as well as in tetraploid wheat varieties T.dicoccum v farum Gli A1dmh and Gli B1dfc were identified. The identification of new allelic blocks of components makes it possible to use them as genetic markers in the future for the certification and breeding of new varieties.
About the Author
H. B. Sadigov
Genetic Resources Institute of Azerbaijan National Academy of Sciences
Azerbaijan
Azerbaijan
Candidate of Science in Biology, Associate Professor, Head of Department.
155 Prospect Svobody, Baku, AZ 1106, Azerbaijan
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Review
For citations:
Sadigov H.B. Hybridological analysis of gliadincoding loci of F2 wheat from interspecific hybrid combination. Siberian Herald of Agricultural Science. 2019;49(1):95-101. (In Russ.) https://doi.org/10.26898/0370-8799-2019-1-12
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