Disinfection of premises in the presence of calves

The paper presents the results of studies on effectiveness of the preparation based on B. subtilis bacteria strains for the disinfection of livestock buildings in the presence of animals. The dynamics of bacterial contamination of livestock buildings was studied using the preparation with an equal combination of B. subtilis TNP-3 and B. subtilis TNP-5 bacteria strains. The work was carried out in the winter at a livestock complex in the Republic of Sakha (Yakutia). For experiments, two experimental and two control sections were organized with six newborn calves in each. The preparation for disinfection was applied by the method of mist spraying. The quality of disinfection was determined after 15 days by the quantity of mesophyll aerobic and facultative anaerobic microorganisms (QMAFAM), bacteria of the Escherichia coli group, staphylococci and microscopic fungi left after washing the fl oor surface in accordance with the approved recommendations. Sanitation of section cells with a preparation containing 1.0 × 109 CFU / cm3 with a fl ow rate of 400 ml / m2 at a temperature of –2 °C to 10 °C inhibited the accumulation of opportunistic microorganisms, including mold and toxigenic fungi. It was noted that QMAFAM decreased by 73.5 times, the number of Escherichia – by 88 times, staphylococci – by 68, fungi of the genus Mucor – by 40 times, Aspergillus fl avus – up to 1.7 × 104 CFU / cm3 in the experimental sections compared to the control. This method of sanitation does not affect the humidity of the livestock building, does not irritate the mucous membranes of the eyes and respiratory tract, and helps to normalize intestinal microbiocenosis. The use of the preparation based on B. subtilis bacteria strains contributes to the prevention of diseases and the preservation of livestock. The method of disinfection in the presence of animals at low positive temperatures in livestock buildings was granted a patent of the Russian Federation for inventions.

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