Evaluation of the individual level of homozygosity of bulls on the basis of genomic information
https://doi.org/10.26898/0370-8799-2019-6-9
Abstract
The results of the evaluation of genomic inbreeding of stud bulls of Black-and-White and Holstein breeds of the Central region of Russia are presented. The studies were carried out by detecting long homozygous fragments in the animal genome – runs of homozygosity (ROH), compared to the traditional approach of determining inbreeding by pedigree. ROH analysis was performed using the Illumina Bovine SNP50K v2 BeadChip biochip with the density of 54609 SNP in the population of stud bulls of Black-and-White and Holstein breeds (n = 100) in Moscow and Leningrad regions. The largest number and total length of ROH in the studied population were found on chromosomes 3, 6, 9, 10, 20, and 24, with an average length of 10.34; 9.15; 10.33; 12.37, and 9.05 Mb, respectively. The number of ROH segments varied from 5 to 34, with an average of 19.1. The average ROH size was 8539 ± 161 kb with the total number of nucleotide mutations of 136.2 ± 2.5 SNP and coverage density of 62.2 kb. It was found that the larger the inbreeding value by pedigree, the higher the frequency of occurrence of homozygous segments (on average from 15.5 to 25.7, respectively, with the inbreeding value from 0 to 8.5%). The total length of the fragments and the average value of ROH tend to increase depending on the inbreeding level (271.3 Mb and 10862 kb at the inbreeding value of 5.0% and higher). The highest repeatability with the inbreeding coeffi cient was obtained for the sum of homozygous genome patterns and diallelic markers, 0.517 and 0.475, respectively. Monitoring the level of homozygosity on the basis of genomic information most accurately refl ects the true level of inbreeding, and allows more effi cient monitoring of the breeding process in animal populations.
About the Authors
L. P. Ignatieva
Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Candidate of Science in Agriculture, Lead Researcher
Moscow region, Podolsk
A. A. Belous
Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Junior Researcher, Postgraduate Student
Moscow region, Podolsk
I. S. Nedashkovsky
Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Junior Researcher, Postgraduate Student
Moscow region, Podolsk
О. V. Kostyunina
Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Doctor of Science in Biology, Head of Laboratory, Lead Researcher
Moscow region, Podolsk
A. A. Sermyagin
Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Candidate of Science in Agriculture, Head of Division, Lead Researcher
60 Dubrovitsy, Podolsk, Moscow region, 142132
N. A. Zinovieva
Federal Science Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Doctor of Science in Biology, Professor, Member RAS academy, Director of Federal Science Center for Animal Husbandry named after L.K.
Moscow region, Podolsk
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Review
For citations:
Ignatieva L.P., Belous A.A., Nedashkovsky I.S., Kostyunina О.V., Sermyagin A.A., Zinovieva N.A. Evaluation of the individual level of homozygosity of bulls on the basis of genomic information. Siberian Herald of Agricultural Science. 2019;49(6):79-87. (In Russ.) https://doi.org/10.26898/0370-8799-2019-6-9
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