COMPARATIVE ANALYSIS OF APPLICATION OF ELISA AND AGID IN DIAGNOSING BOVINE LEUKOSIS
Abstract
Results are given from a comparative analysis of the two methods for diagnosis - agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) - used in carrying out sanitation measures against bovine leukosis. Diagnostic tests for BLV infections were conducted using the facilities of the Leukosis Laboratory at the Institute of Experimental Veterinary Science of Siberia and the Far East, Novosibirsk. The comparative analysis was carried out on large cattle population from livestock farms with various rates of the spread of bovine leukosis. It has been found that the ELISA test practically always reveals animals not detected by AGID. Advantages of ELISA over AGID are shown. The higher sensitivity rates in ELISA allow detecting up to 15.3% of virus carriers in addition to the AGID results. In ELISA, both individual and combined samples of milk and blood serum can be used as the research material. A procedure of carrying out the analysis and recording results can be automated. Results of ELISA tests can be obtained within a few hours unlike AGID tests, where it takes several days. It has been concluded that the ELISA technique is more expensive as compared with AGID but, thanks to its advantages, reduces rate of tests and terms of sanitation of cattle herds against leukosis.
About the Authors
N. G. Dvoeglazov
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
V. V. Khramtsov
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
T. A. Agarkova
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
N. A. Osipova
Institute of Experimental Veterinary Science of Siberia and the Far East
Russian Federation
Russian Federation
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Review
For citations:
Dvoeglazov N.G., Khramtsov V.V., Agarkova T.A., Osipova N.A. COMPARATIVE ANALYSIS OF APPLICATION OF ELISA AND AGID IN DIAGNOSING BOVINE LEUKOSIS. Siberian Herald of Agricultural Science. 2015;(1):89-93. (In Russ.)
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