Polyphenolic compounds of blueberry (Vaccinium uliginosum L.) from the Magadan expedition identified by tandem mass spectrometry

A mass spectrometric study to clarify the polyphenolic composition of blueberry Vaccinium uligi nosum L. berries sampled at eight geographical locations in the Magadan region during the expedition in August 2023 is presented. The method of tandem mass spectrometry (high-performance liquid chromatography coupled with an ion trap) was applied in the study, which allows to identify with high accuracy the composition of chemical compounds of the studied object. Chemical compounds were identified by comparing their retention index, mass spectra and MS fragmentation with an in-house database based on the data from other spectroscopic techniques such as nuclear magnetic resonance, ultraviolet spectroscopy and mass spectrometry, as well as the data from scientific literature. The presence of 134 compounds was identified, of which 114 compounds represent the group of polyphe nols with 20 additional compounds of other chemical groups being also identified. 46 compounds of polyphenolic group have been identified for the first time in the Vaccinium uliginosum berry extracts (5 flavones, 13 flavanols, 6 flavan-3-ols, 10 anthocyanins, 5 phenolic acids, 2 stilbene, 4 couma rin, 1 lignan). These are the flavones formononetin, nepetin, cirsilineol, herbacetin, syringetin; flavo nols isoramnetin, rhamnetin II, myricetin, mersetin, avicularin, isoramnetin 3-O-glucoside, myricetin 3-O-glucoside; flavan-3-ols afzelechin, epiafzelechin, gallocatechin, epigallocatechin; a large group of anthocyanins: delphinidin O-pentoside, delphinidin 3-O-xyloside, delphinidin 3-O-hexoside, malvi din pentoside, malvidin 3-O-hexoside, dihydrochalcone ploretin, 2 stilbenes (3-hydroxyresveratrol and resveratrol), 4 coumarins (umbelliferone, scopoletin, fraxetin, umbelliferone hexoside), lignan syringarezinol, etc. Compounds of other chemical groups identified for the first time in the Vaccinium uliginosum berry extracts are stearidonic acid, myristoleic acid, linolenic acid, sebacic acid, glucaric acid, shikimic acid, vebonol, sespendol. In total, the newly obtained knowledge allows expanding the use of Vaccinium uliginosum extracts both for various nutritional and pharmaceutical purposes, as well as in the development of new effective dietary supplements to maintain public health.

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