DIFFERENCES IN DESIRED GENOTYPES OF THE PRLR GENE IN PIGS OF DIFFERENT BREEDS
Abstract
Results are given from studies on the effect of polymorphism in the prolactin receptor gene (PRLR) onreproductive performance of purebred Landrace and Large White and F1 crossbred pigs. The PRLR gene in pigs is localized in chromosome 16 (SSC16). The presence of polymorphism is associated with phenotypic variation in reproductive traits of pigs. The studies were carried out on Large White (n=71), Landrace (n=116), and crossbred (n=130) pigs, which were obtained by crossing _ Landrace _ _ Large White under on-the-farm conditions in Russia. The nuclear DNA of the pigs was extracted from 50 mcg of a pre-prepared sample using a K-Sorb-100 reagent kit. The restriction of the amplified fragment was carried out with the AluI endonuclease. The size of the restriction fragments obtained was determined by the electrophoresis method in a 2.5% agarose gel with the addition of ethidium bromide. The presence of polymorphism was found to be in all breeds studied. The analysis of reproductive performance of pigs has shown that Landrace sows with the best reproductive traits are associated with the AA/PRLR genotype. The presence of this genotype is associated with the greater number of piglets, multifetation, and litter birth weight as compared with the BB/PRLR genotype. In Large White pigs,positive effects were established in animals of the BB/PRLR genotype. In crossbred pigs, the best reproductive performance is associated with the AB/PRLR genotype. The breed-specific effect of the PRLR polymorphism is observed in the studies, which is of interest in obtaining F1 pigs used in the first stage of hybridization.
About the Authors
A. I. KLIMENKO
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Member of the Russian Academy of Sciences, Head Researcher
A. YU. KOLOSOV
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Candidate of Science in Agriculture, Senior Researcher
M. A. LEONOVA
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Candidate of Science in Agriculture, Senior ResearcherL. V. GETMANTSEVA
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Candidate of Science in Agriculture, Laboratory HeadS. YU. BAKOEV
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Candidate of Science in Biology, Senior ResearcherA. V. RADYUK
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Junior ResearcherE. A. ROMANETS
Don State Agrarian University
Russian Federation
Russian Federation
24, Krivoshlykova St, Persianovsky, Oktyabrsky District, Rostov Region, 346493, Russia
Master’s Degree StudentReferences
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For citations:
KLIMENKO A.I., KOLOSOV A.Yu., LEONOVA M.A., GETMANTSEVA L.V., BAKOEV S.Yu., RADYUK A.V., ROMANETS E.A. DIFFERENCES IN DESIRED GENOTYPES OF THE PRLR GENE IN PIGS OF DIFFERENT BREEDS. Siberian Herald of Agricultural Science. 2017;47(4):32-37. (In Russ.)
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