STUDYING ACTIVATION OF A PHOTOSENSITIZER BY RADIATION WITH NON-SPECIFIC WAVELENGTH
Abstract
Results are given from studies in the field of photodynamic therapy on diagnosing and treating malignant neoplasms. There was studied the efficiency of activation of 0.02% and 0.1% methylene blue solutions by specific optical radiation with the wavelength of 620 nm and nonspecific optical radiation with the wavelength of 405 nm. We used LED sources of monochrome unpolarized optical radiation with wavelengths of 620 nm and 405 nm and output power of 3,500 milliwatts each. The efficiency of solution activation was evaluated by voltage on copper electrodes immersed in the photosensitizer solution, which was measured by an analog oscilloscope. The indices were recorded every 30 seconds until the maximum voltage on the electrodes was reached, then 0.5 g of organic matter (meat peptone agar) was added to the activated photosensitizer solution. Optical radiation with the wavelength of 620 nm was found to cause the stable activation of the photosensitizer, in contrast to radiation with the wavelength of 405 nm, the effect of which was expressed directly during irradiation. The concentration of the photosensitizer solution had a direct effect on the activation efficiency; so, the activation in 0.2% methylene blue solution was more pronounced than that in 0.1%, which can be explained by lower optical density of the former. The introduction of organic matter was accompanied by a sharp increase in the voltage at the electrodes. Thus, optical radiation with the wavelength of 405 nm, which is nonspecific for methylene blue as a photosensitizing dye, can be used to activate it.
About the Authors
A. V. PAVLOV
Institute of Experimental Veterinary Science of Siberia and the Far East, SFSCA RAS
Russian Federation
Russian Federation
Krasnoobsk, Novosibirsk Region, 630501, Russia
Candidate of Science in Biology, Senior Researcher
Е. СМЕРТИНА
Institute of Experimental Veterinary Science of Siberia and the Far East, SFSCA RAS
Russian Federation
Russian Federation
Krasnoobsk, Novosibirsk Region, 630501, Russia
Doctor of Science in Veterinary Medicine, Laboratory Head
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Review
For citations:
PAVLOV A.V., STUDYING ACTIVATION OF A PHOTOSENSITIZER BY RADIATION WITH NON-SPECIFIC WAVELENGTH. Siberian Herald of Agricultural Science. 2017;47(4):54-59. (In Russ.)
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