EFFECTIVENESS OF USING ENZYMES OF MICROBIAL ORIGIN IN MARAL RAW STUFF PROCESSING
https://doi.org/10.26898/0370-8799-2017-5-12
Abstract
Effectiveness of using enzymes of microbial origin in deep processing of maral raw stuff was studied. There was conducted comparative evaluation of using enzymes Protamex, Protease 1, Protease 2, Protomix, and Bacterial Protease when enzyme hydrolyzing by-products of maral farming – tails, reproductive organs of males, uteri with embryos, tendons. The enzymatic hydrolysis was carried out during 8–10 hours in the thermostat and the ultrasonic field at a temperature of +45 to +50oC. It has been found that the use of the enzymes studied and their combinations allows us to increase a transition rate from dry substances to hydrolyzates. When adding each enzyme separately during the hydrolysis of maral by-products, best results as to output of soluble components were obtained with Protomix and Bacterial Protease. At all stages of the study, the maximum outputs of concentrate were obtained due to the use of enzyme combinations. The addition of Protease 2 and Protomix during the fermentation of maral tails increased a proportion of soluble components up to 98%. When hydrolyzing the reproductive organs of males in the presence of Protease 1, Protease 2 and Protomix, the output of concentrate (dried to a moisture content of 10–12% hydrolyzate) increased to 65.2%. The use of Protomix and Papain combination in the hydrolysis process of the uteri with embryos and tendons resulted in the values of 75.3 and 71.0%, respectively. As shown by research, the use of ultrasonic vibrations allowed increasing the concentrate output by 10.8–21.2% as compared with the hydrolysis of raw material in the thermostat. Therefore the use of ultrasound in combination with the enzymes of microbial origin studied is effective for hydrolysis of maral raw stuff.
About the Authors
M. G. KROTOVA
All-Russian Research Institute for Antlered Deer Farming
Russian Federation
Russian Federation
Researcher
160, Shevchenko St, Barnaul, Altai Territory, 656031, Russia
V. G. LUNITSYN
All-Russian Research Institute for Antlered Deer Farming
Russian Federation
Russian Federation
Doctor of Science in Veterinary Medicine, Director
160, Shevchenko St, Barnaul, Altai Territory, 656031, Russia
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Review
For citations:
KROTOVA M.G., LUNITSYN V.G. EFFECTIVENESS OF USING ENZYMES OF MICROBIAL ORIGIN IN MARAL RAW STUFF PROCESSING. Siberian Herald of Agricultural Science. 2017;47(5):97-103. (In Russ.) https://doi.org/10.26898/0370-8799-2017-5-12
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