Preview

Siberian Herald of Agricultural Science

Advanced search

SORPTION ACTIVITY OF MANNAN OLIGOSACCHARIDES AGAIST MICROORGANISMS OF THE GENUS SALMONELLA IN EXPERIMENTS IN VITRO AND IN VIVO

Abstract

There are presented studies on using a feed additive containing mannan oligosaccharides to ensure the biological purity of poultry products. Mannan oligosaccharides (MOS) are natural chemical compounds derived from cell walls of the yeast Saccharomyces. In vitro studies of the sorption activity of the feed additive containing mannan oligosaccharides were conducted on museum strains and field isolates of microorganisms of the genus Salmonella. Data resulting from research indicate that the feed additive has pronounced sorption activity against microorganisms Salmonella spp. (63.98 percent). To study the effect of mannan oligosaccharides on the contamination of the muscles and internal organs by microorganisms Salmonella spp., 5 groups in 15 of day-old egg-type chicks were formed. The chicks of the experimental groups were fed a diet with MOS at concentrations of 0.1; 0.5; 1 and 2 percent of feed weight. The chicks of the control group were fed a diet with antibiotics instead of MOS. To determine a contamination level in the muscles and internal organs of chicks by microorganisms Salmonella spp., on the 30th day of the experiment was made a post-slaughter examination and sampling of muscles, liver, small and large intestinal contents. It has been established that the daily application of the feed additive, containing MOS at the concentration of 2 percent of the feed weight, reduces the bacterial contamination of chicks’ carcasses and internal organs by microorganisms of the genus Salmonella by 76.6 percent, as compared with the control. The use of MOS allow us to improve the biological safety of poultry products owing to decreased part of feed antibiotics in a diet and reduction in contamination level in the muscles and internal organs of chicks.

About the Authors

V. YU. Koptev
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation


N. A. Shkil
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation


M. A. Titova
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation


I. S. Onishchenko
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation


N. YU. Balybina
Institute of Experimental Veterinary Science of Siberia and the Far East, Russian Academy of Agricultural Sciences
Russian Federation


A. L. Bychkov
Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of the Russian Academy of Sciences
Russian Federation


References

1. Бакулин В.А. Болезни птиц. - СПб., 2006. - С. 285-295.

2. Болезни домашних и сельскохозяйственных птиц / под ред. Кэлнека и др. - М.: Аквариум Бук, 2003. - С. 119-147.

3. Куриленко А.Н., Крупальник В.Л., Пименов Н.В. Бактериальные и вирусные болезни молодняка сельскохозяйственных животных. - М.: КолосС, 2005. - С. 28-44.

4. Белоусова Н.И., Гордиенко С.В., Ерошин В.К. и др. Получение смесей аминокислот на основе автолизатов дрожжей Saccharomyces, выращенных на эталоне или сахарах // Биотехнология. - 1990. - № 3. - С. 6-9.

5. Bychkov A.L., Korolev K.G., Lomovsky O.I. Obtaining mannanoligosaccharide preparations by means of the mechanoenzymatic hydrolysis of yeast biomass // Applied Biochemistry and Biotechnology. - 2010. - Vol. 162. - P. 2008-2014.

6. Скородумов и др. Микробиологическая диагностика бактериальных болезней животных. - М.: Изографь, 2005. - С. 388-413.


Review

For citations:


Koptev V.Yu., Shkil N.A., Titova M.A., Onishchenko I.S., Balybina N.Yu., Bychkov A.L. SORPTION ACTIVITY OF MANNAN OLIGOSACCHARIDES AGAIST MICROORGANISMS OF THE GENUS SALMONELLA IN EXPERIMENTS IN VITRO AND IN VIVO. Siberian Herald of Agricultural Science. 2014;(2):47-52. (In Russ.)

Views: 447


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 0370-8799 (Print)
ISSN 2658-462X (Online)