Calcium as an inhibitor of tripsin activity in pancreatic juice of chicken
https://doi.org/10.26898/0370-8799-2021-2-10
Abstract
The results of studying the interaction of calcium and pancreatic enzymes in 46-week-old Hisex white chickens are presented. It is shown that intestinal absorption of calcium is accompanied by endogenous excretion of calcium with digestive juices. The effect of calcium on tryptic activity in an in vitro experiment was determined. In the experiment, different doses of calcium (5.0; 7.5; 0.5; 12.5 and 25.0 mg) were injected into the pancreatic juice of chickens, previously diluted with physiological solution 10 times. The number of repetitions in each variant of the experiment was 20 times. For the experiment, 2-aqueous calcium chloride (Chimmed, RF) was used. Trypsin activity was determined by the kinetic method. The content of calcium was determined with a Sinnowa BS-3000P biochemical analyzer (China) and a set for the determination of calcium in the blood of animals DIAKON-VET (RF). It was found that pancreatic juice of chickens contains up to 2.9 ± 0.03 mmol of calcium/l, which is comparable to the level of calcium in the blood serum of 1.99 ± 0.10 – 3.13 ± 0.20 mmol/l. The inhibiting effect of calcium on the tryptic activity was found. A five-fold increase in calcium in pancreatic juice of chickens reduces the activity of trypsin by 34.7%. According to the analysis of variance, the concentration of calcium in pancreatic juice affects tryptic activity, the reliability of the effect is confirmed by 92%. A stable negative correlation was established between calcium content in pancreatic juice and activity of trypsin r = –0.78, which is consistent with the correlation of the corresponding parameters in the blood. These findings serve as evidence that calcium together with proteases can be regarded as an element that regulates metabolic processes in chickens.
About the Authors
V. G. Vertiprakhov
Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of the Russian Academy of Sciences
Russian Federation
Russian Federation
Vladimir G. Vertiprakhov - Doctor of Science in Biology, Head Researcher, Head of Dept.
10, Ptitsegradskaya St., Sergiev Posad, Moscow Region, 141300.
A. A. Grozina
Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of the Russian Academy of Sciences
Russian Federation
Russian Federation
Alena A. Grozina - Candidate of Science in Biology, Lead Researcher.
10, Ptitsegradskaya St., Sergiev Posad, Moscow Region, 141300.
V. I. Fisinin
Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of the Russian Academy of Sciences
Russian Federation
Russian Federation
Vladimir I. Fisinin - Doctor of Science in Agriculture, Professor, RAS Academician, Scientific Supervisor.
10, Ptitsegradskaya St., Sergiev Posad, Moscow Region, 141300.
I. V. Kislova
Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of the Russian Academy of Sciences
Russian Federation
Russian Federation
Irina V. Kislova - Junior Researcher.
10, Ptitsegradskaya St., Sergiev Posad, Moscow Region, 141300.
N. V. Ovchinnikova
Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of the Russian Academy of Sciences
Russian Federation
Russian Federation
Natalia V. Ovchinnikova - Junior Researcher.
10, Ptitsegradskaya St., Sergiev Posad, Moscow Region, 141300.
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For citations:
Vertiprakhov V.G., Grozina A.A., Fisinin V.I., Kislova I.V., Ovchinnikova N.V. Calcium as an inhibitor of tripsin activity in pancreatic juice of chicken. Siberian Herald of Agricultural Science. 2021;51(2):80-86. https://doi.org/10.26898/0370-8799-2021-2-10
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