Creation of experimental samples of a probiotic preparation for farm animals and measurement of vitamin B₁₂ content in them

In modern realities the idea of healthy nutrition is of particular importance, this interest increases the demand of consumers for products that are ecologically clean and complete in their composition. In this regard, reduction of antibiotic use frequency and active application of probiotic additives in the main feed rations are becoming topical issues of livestock breeding. Also integral to the industry are the preservation of young stock and increasing the productivity of the herd. The most important step in creating a probiotic is choosing its composition. The most common components of probiotic supplements are lactobacillus and propionic acid bacteria, as these microorganisms have the necessary set of technologically valuable properties. Propionic acid bacteria are producers of vitamin B₁₂ , which has a positive effect on protein metabolism and increases feed digestibility. Animals do not fully cover the need for vitamin B₁₂ , so it becomes necessary to include it in the composition of the feed additives. Lactobacillus have high biological and antagonistic activity and exert immunostimulatory effect. During previous and current studies conducted on the basis of the Siberian Research Institute of Cheese Making, the optimal nutrient medium for accumulation of biomasses of the above microorganisms was selected, as well as the optimal dose of their introduction into the probiotic preparation. Based on these data, three experimental samples of biopreparation for farm animals have been formulated. Both co-cultivation and separate cultivation were applied in the process of specimen creation. The study also determined the active acidity and vitamin B₁₂ content of all the samples. Vitamin B₁₂ content ranged from 0.70 ± 0.01 to 0.82 ± 0.03 μg/ml. As a result of the work done, it has been found that sample  No. 1 is the most optimal in terms of processability, as well as the quantitative content of vitamin B₁₂ .

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