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Effect of plant lactones and flavonoids on intestinal microflora structure and fatty acid concentration in cattle
https://doi.org/10.26898/0370-8799-2025-5-8
Abstract
The effect of plant composition of common wormwood (Artemisia absinthium) alone and in combination with cobalt (II) chloride in chelate form (CoCl2 l) on the changes in the taxonomic profile of rumen microbiome of ruminants in the in vivo experiment was evaluated. The studies determined the changes in the bacterial composition of rumen fluid by using the sequencing method on the MiSeq device. The object of the research were young bulls (n=4) of the Kazakh White-headed breed at the age of 12–13 months with an average live weight of 336–340 kg. During the study, animals in the experimental groups were given Artemisia absinthium herbal (A. absinthium) and cobalt (II) chloride (CoCl2 l): experimental group I – A. absinthium at a dose of 2.0 g/kg dry matter, experimental group II – A. absinthium in dose of 2.0 g/kg DM with additional CoCl2 l (1.5 mg/kg/DM), experimental group III – only CoCl2 l (1.5 mg/kg/DM). The preparatory period lasted for 14 days; the main period was 7 days. At the end of the baseline period, rumen contents were sampled through a fistula to analyze fatty acid concentrations. Samples of intestinal contents were taken into sterile containers by rectal palpation, and for metagenomic sequencing – into 1.5 ml Eppendorf microtubes. Data analysis showed that administration of A. absinthium and cobalt (II) chloride in the form closest to the natural one was able to modulate the intestinal microbial community, affecting the concentration and assimilation of fatty acids. The results obtained indicate the potential of using A. absinthium and (CoCl2 l) compositions in cattle nutrition as a natural alternative to improve ruminant production systems.
Supporting agencies: This work was carried out with financial support from the Russian Science Foundation (project No. 21-76-10014).
About the Authors
V. A. Ryazanov
Federal Research Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Russian Federation
Russian Federation
Vitaly A. Ryazanov - Senior Researcher, Candidate of Science in Agriculture
Orenburg
G. K. Duskaev
Federal Research Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Russian Federation
Russian Federation
Galimzhan K. Duskaev - Deputy Director, Lead Researcher, Doctor of Science in Biology, Associate Professor, Professor RAS
Orenburg
M. Ya. Kurulkina
Federal Research Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Russian Federation
Russian Federation
Marina Ya. Kurulkina - Senior Researcher, Candidate of Science in Biology
address: 29, January 9th St., Orenburg, 460000
D. M. Muslyumova
Federal Research Center of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Russian Federation
Russian Federation
Dina M. Muslyumova - Senior Researcher, Candidate of Science in Biology
Orenburg
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For citations:
Ryazanov V.A., Duskaev G.K., Kurulkina M.Ya., Muslyumova D.M. Effect of plant lactones and flavonoids on intestinal microflora structure and fatty acid concentration in cattle. Siberian Herald of Agricultural Science. 2025;55(5):83-98. (In Russ.) https://doi.org/10.26898/0370-8799-2025-5-8
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