The results of research on clonal micropropagation of 17 raspberry varieties of the Altai and European selection are presented. Recently, researchers have been focusing on creating a universal nutrient medium that would be equally suitable for any variety of a particular plant species. This is especially important for mass clonal micropropagation in production nurseries. The regeneration potential of the raspberry varieties of different types of fruiting at each stage of reproduction has been assessed. At the stage of actual micropropagation, it was found that Driver and Kuniyuki medium in our modification was superior to the Murashige and Skoog medium in terms of the shoot length and averaged 9.0 mm for common type varieties and 12.3 mm for remontant varieties. This made it possible to eliminate the additional stage of elongation of the microshoots. Also, the appearance of microshoots improved on the new medium. It was found that for raspberry, regardless of its type, the optimal concentrations of the growth regulators lie in the following range: cytokinin 6-benzylaminopurine (BAP) 2.5–3.5 μM together with auxin ß-indolyl butyric acid (IBA) 0.5–0.7 μM. At the stage of rhizogenesis, the best results were shown by the Driver and Kuniyuki medium with 2-fold reduced content of all main components and with the addition of 2.0 μM IBA at the passage duration of 21 days. After that, all microcuttings with and without roots underwent the adaptation stage – growth completion in neutral substrates for 56 days under ex vitro conditions in a vegetation room. In most cases, there were no statistically significant differences in the length and number of leaves regardless of the presence or absence of roots at the initial stage of adaptation. By the end of this stage, 100% of the adapted raspberry plants of the usual type of fruiting and 98% of the remontant type were obtained.

The results of studying potato hybrids in the nurseries of preliminary and competitive variety trials are presented. The research was conducted in 2018–2022 in the Krasnoyarsk Territory in grain and fallow crop rotation. The soil of the experimental plot is represented by leached chernozem, low- and medium-moist heavy loam of granulometric composition. Experiment design, yield recording and data processing were carried out in accordance with the generally accepted recommendations for work with the crop. Based on the Selyaninov hydrothermal coefficient, the weather conditions of the vegetation periods of the years of research can be characterized as slightly arid.   The HTC was 1.1 in 2019 and 2021, in 2020, 2022 – 1,3. According to the results of the preliminary variety trials, lines A-114, A-326, A-529, A-635a exceeded or were at the level of the standard variety Krasnoyarsky Ranniy by the number of marketable tubers and yield. During 4 years of research in the nursery of competitive varietal trial, the studied breeding lines on average formed yields at the level or higher than the standard varieties. According to the elements of the yield structure in the competitive variety trial lines A-114, A-529 significantly exceeded the standard variety in marketability, as well as had a smaller number and weight of unmarketable tubers. Based on the results of the evaluation in 2019–2021 of the chemical composition of tubers for dietary and functional food, lines with low starch content (up to 14%) are proposed: A-114 (12.2%), А-672^а (9.9), А-635^а (12.7%). Based on the study in 2019–2022 of the line A-114 in competitive trials, preliminary and production multiplication, ecological assessment, application materials for admission to use and protection of the new potato variety Akrux were submitted to the State Variety Commission.

The paper presents the results of evaluation of the KASIB-22 nursery soft wheat samples resistance to the main wheat pathogens. The research material was 44 wheat samples obtained from different regions of Russia and Kazakhstan within the framework of the KASIB shuttle breeding program (A.I. Barayev Research and Production Centre for Grain Farming, Omsk State Agrarian University, ICiG SB RAS, Karabalyk AES, Karaganda AES, North-Kazakhstan AES, FSBSI "Omsk ASC", HE SAU of the Northern Trans-Urals, Aktyubinsk AES, Kurgan Research Institute of Agriculture, Pavlodar AES, agrokomplex "Kurgansemena", Samara Research Institute of Agriculture, Chelyabinsk Research Institute of Agriculture). The following breeding lines were identified as highly productive and possessing high and medium resistance to brown rust, powdery mildew and loose smut: Line Pt-311 (Kurgan Research Institute of Agriculture), Lutescens 1462, Lutescens 1486, Line1616ae14 (Samara Research Institute of Agriculture), Lutescens 1356 (ICiG SB RAS). The yield of the selected samples averaged 42.1–51.2 c/ha. Genotypes Novosibirskaya 41, Line 435/12 and Lutescens 1364 are of interest for selection for quality. The gluten content of the nursery genotypes ranged from 24.1 (Line 23/07 in 2022) to 38.0% (variety Astana 2 in 2021), protein ranged from 13.4 (variety Tertia in 2022) to 19.5% (Lutescens 1364 in 2022). Each of the selected lines is of practical value for breeding, and the samples from the KASIB-22 nursery allow to expand the genetic diversity of spring soft wheat by selecting the most resistant forms to local populations of powdery mildew, leaf rust and loose smut pathogens.

The results of the study of architectonics and physical and mechanical properties of the stems of selective forms of spring hexaploid triticale of different ecological and geographical origin are presented. Traits of 19 collection and breeding samples from the collections of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) and the Siberian Research Institute of Plant Growing and Selection - branch of the Federal Research Center of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences (SibNIIRS – branch of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences) served as research objects. Macrometric parameters were studied on 25 representative specimens of each breeding sample when they reached the phase of full ripeness during three years (2021–2023). The resistance of the stem to bending or fracture was determined on an experimental specimen manufactured at the SibNIIRS with a stable platform, bars for fixing the specimen and a slot for placing a dynamometer. The susceptibility of triticale plants to lodging was evaluated by a set of traits: weight of a 10-cm straw of the 2nd internode; stem resistance to breaking determined by the peak reading of an electronic dynamometer; stem tissue density of the 2nd internode. The studied samples differed significantly in these parameters and were characterized by different degrees of the resistance to lodging. Promising breeding specimens that can be used as sources of valuable traits have been identified based on the totality of the strength parameters. Application of the principal component method allowed to identify the marker trait "sum of lengths of the 2nd and 3rd internodes", which was determinant in providing high lodging resistance in the studied samples. A close relationship between the trait of 10-cm straw mass of the 2nd internode and the parameter of the stem resistance to breakage has been established. The conjugation of these characteristics will make it possible to identify the sources of valuable traits and purposefully carry out the selection process of triticale for resistance to lodging without attracting short-stemmed forms.

The analysis of variability of winter soft wheat lines by quantitative traits is presented. The research was conducted in 2020–2023 in the Rostov region. Seven lines of winter soft wheat of the competitive variety trial were taken as an object of research. The Ermak variety was used as a standard. The yields of the lines during the years of study averaged from 9.78 to 10.12 t/ha with an average of 9.76 t/ha in the experiment. Plant height ranged from 85.0 to 115.0 cm. All the lines studied were classified as small- to medium-grained (32.9–45.9 g) in terms of 1000 grain weight. On average for the years of study, the minimum mass of 1000 grains was formed by the line 1638/19 (34.6 g), and the maximum – by the line 1361/19 (43.5 g). The lines had different groups of ripeness according to the indicator of the earing date. The spikelet length varied from 7.3 to 10.0 cm. The highest spikelet length was formed by the line 1361/19 (10.0 cm), the shortest – by the line 1120/19 (7.3 cm). The number of productive stems ranged from 471 to 924 stems/m². The maximum number of productive stems for the years of the study was formed by the line 1921/20 (758 pcs./m²), the minimum –  by the line 1361/19 (689 pcs./m²). The number of grains per plant ranged from 61.6 to 110.6. On average, the highest number of grains per plant was formed by the line 1638/19 (100.6 pcs.), the lowest – 1343/19 (70.4 pcs.). The grain weight per plant averaged 3.54 g and ranged from 2.26 to 4.56 g. The number of grains per ear varied from 28.9 to 51.5 pieces, the maximum number was formed by the lines 1361/19 (42.7 pcs.) and 1638/19 (41.2 pcs.). In terms of the grain weight per ear the standard was exceeded by the line 1361/19 (1.93 g), the values for the indicator varied from 1.02 to 2.28 g.

The results of the analysis of the yield and biological efficiency in the joint cultivation of festulolium and esparcet with various methods of sowing and applying nitrogen fertilizers in spring in the conditions of the forest-steppe zone of Western Siberia are presented. The survival rate of festulolium plants after overwintering the first year of life was 86%, esparcet survival rate in single-species sowing was 86% and decreased when using the skip-row planting to 76%, when sowing with a mixture of seeds to 67%. Joint sowing of festulolium with esparcet increased the yield by 22.6% compared with single-species sowing of festulolium (30.38 t/ha of the green mass) when using the skip-row planting and by 7.9% (to 26.73 t/ha) when sown with a mixture of seeds. The energy efficiency ratio (EER) is 7.7–9.0 and is characterized as high, the profitability of the mixtures is 373%. The introduction of mineral nitrogen at a dose of 30 kg a.i./ ha significantly increased the yield of the mixtures of festulolium with esparcet in the variants skip-row planting by 29.5% and in the variant of sowing with a mixture of seeds by 11.5% due to an increase in the leafiness of the legume component. In the experiment the EER equals 4.4–5.2 and is characterized as high, the profitability of sowing the mixtures is 352%. With an increase in the dose of mineral nitrogen to 60 kg a.i. / ha, the maximum yield on the variant of sowing with a mixture of seeds is 41.84 t / ha of green mass, which is 32% higher than single–species sowing of festulolium. The increase is due to a 4% increase in the height of the festulolium plants, and 60% of its mass. When using skip-row planting, the yield was 39.11 t/ha of the green mass, which is 23.4% higher than the control. The EER equals 4.7–4.9 and is characterized as average, the profitability of sowing the mixtures is 349–364%.

The arable land efficiency ratio (LER) in the mixtures was 0.96–1.06, which confirms their effectiveness.

For the first time in Western Siberia (Novosibirsk region), two-year production trials of an experimental prototype of a drug based on the entomopathogenic fungus Metarhizium robertsii have been conducted. Strain P-72 isolated from the dead larvae of Colorado potato beetle Leptinotarsa decemlineata Say from the collection of microorganisms of the Institute of Systematics and Ecology of Animals of the Siberian Branch of the Russian Academy of Sciences was used in this work. Species identification of the fungus was performed based on sequencing of the elongation factor gene region EF1α. The ability of the preparation to significantly increase the parameters of potato yield structure elements throughout the whole vegetation period was shown. A reliable increase in the number of surviving sprouts by 1.4 times, the number of stems and tubers by 1.5 times was found. The growth-stimulating effect of using the preparation based on entomopathogenic fungus was manifested in the increase of plant weight by the flowering phase. The average yield increase for two years amounted to 36.0% in the variant with application of M. robertsii significantly exceeding the productivity of potatoes in the control. The level of profitability of potato production with the use of entomopathogenic fungus M. robertsii increased to 92.0% (with 50.0% in the control) due to the increase in gross yield and improved quality of marketable products. Infestation of new harvest tubers with rhizoctoniose significantly decreased in the variant including treatment with entomopathogenic fungus: the number of tubers with sclerotia decreased 2.8 times compared to the control, which eventually led to an increase in the number of healthy tubers and a significant (2.5 times) decrease in the sclerotial index.

In the course of the experiment, the influence of new feed additives created on the basis of Gaprin and representing biomass of inactivated cell culture of non-pathogenic methane-oxidizing yeasts or bacteria on sturgeon fish productivity indicators was studied. The research was carried out in the Krasnodar Territory on sterlet fingerlings. The experiment lasted for 60 days. According to the scheme of the experiment, the 1st (control) group of fish received complete feeds without additives, i.e. containing 100.0% fish meal. In group 2 feeding, 50.0% fishmeal was replaced with 50.0% Gaprin. Group 3 diet contained 25.0% fishmeal and 75.0% Gaprin. In the feed for group 4, fish meal and Gaprin were in the ratio of 75/25%. In Group 5, fish meal was completely replaced by Gaprin, in this case the amount of additive was 10.5 kg. Sterlet fingerlings from Group 6 received feed with 10.0% Gaprin. The initial live weight in all groups was similar at 30 g. However, the inclusion of Gaprin in the diet by reducing the amount of fish meal contributed to an increase in the final live weight of sterlets in the experimental groups by 4.4–19.4% compared to the control. The exceptions were groups 5 and 6, which received 100.0 and 90.0% Gaprin instead of fishmeal. Final live weight in these groups was lower by 2.3 and 0.9%, respectively. Feed costs per 1 kg of live weight gain in the groups with partial or complete replacement of fish meal were lower relative to the 1st group (from 7.0 to 17.9%,).

Inclusion of Gaprin in complete feeds contributed to the increase in profit from 553.00 to 1785.00 rubles.

The microsatellites polymorphism was analyzed in ewe lambs obtained from intra-breed mating of the Pechora semifine-wool sheep and their littermates from crossing with the Kuibyshev rams. Genotyping of the population by STR loci was performed using the COrDIS Sheep reagent kit recommended by the International Society of Animal Genetics (ISAG). The age of the beginning of reproduction, fertility, milk yield, safety and growth intensity of the litter up to three months of age were taken into account in the ewe lambs. In the group of the Pechora sheep sows 74 alleles were detected, and 81 alleles were detected in the crossbred ones. INRA005 and INRA023 loci were the most polymorphic in both groups, while ETH152 was the least polymorphic. The mean genetic distance between the groups for the 12 STR loci was 0.019 ± 0.003. Pechora sheep (P) and their litters with the Kuibyshev rams (1/2  P + 1/2 K) differed significantly in the frequencies of some STR loci genes. Thus, the INRA023²¹² allele was found with a frequency of 0.174 in the group of P ewes and was absent in the 1/2 P + 1/2 K group, while the INRA172¹²⁶ allele was found with a frequency of 0.326 and 0.122, respectively. The mean Fis values in both groups were close to zero. The groups did not differ significantly in the main genetic-population parameters. However, some advantage of the Pechora ewe sows in comparison with the crossbred sheep 1/2 P + 1/2 K on selective traits, apparently, is due to a higher genetic contribution to their heredity of the indigenous northern short-tailed sheep. According to the results of the study, it can be assumed that the age of onset of reproduction of the ewe lambs is related to the level of their heterozygosity for STR loci.

Multidrug-resistant bacteria have become one of the most serious threats to public health worldwide. The abuse of antibiotics has contributed to the emergence and transmission of resistance mechanisms among bacteria, jeopardizing the therapeutic potential of antibiotics. Uncontrolled use of drugs leads to the formation of antibiotic resistance due to mutations in chromosomal DNA, as well as the production of plasmids, integrons from other bacteria during horizontal gene transfer. In 2010, the BRICS countries (Brazil, Russia, India, China and South Africa) accounted for 76% of antibiotic consumption, with India consuming 12.9 billion units and China – 10 billion units. As of 2017, carbapenem-resistant Acetobacter baumannii and Enterobacteriaceae resulted in approximately US$ 281 million in healthcare costs in the United States. According to numerous reports from the Center for Disease Control and Prevention, approximately 2.3 million episodes of multidrug-resistant microbial diseases resulting in 25,000 deaths are recorded annually in the United States alone. In this regard, the world community of scientists has intensified the study of the combined use of various antibacterial drugs to achieve maximum bactericidal activity. Studies have been conducted to determine the synergistic effect when using combinations of drugs of various pharmacological groups and silver nanoparticles. A significant increase in bactericidal activity by 53.87 times (from 2.528 to 0.0098 mcg/ml) was found with the combined use of AgNPs and DSMO against the reference strain Staphylococcus aureus ATCC 25953. While the cultivation of St. aureus isolate with DSMO and silver nanoparticles revealed a sensitivity increase of 128.2 times (from 5.056 to 0.039 mcg/ml).

The review presents up-to-date information on the genes of resistance of soft wheat (Triticum aestivum L.) to Septoria blight (STB), the causative agents of which are Zymoseptoria tritici, Paras- tagonospora nodorum, Mycosphaerella graminicola. The main attention is paid to the genetic control of wheat resistance to septoria blight from the point of view of the quantitative and qualitative nature of resistance and its effectiveness. The successes achieved so far in the identification of genes and loci of quantitative wheat traits associated with resistance to Z. tritici and P. nodorum are summarized. The characteristics of 15 genes of resistance to septoria blight and the most commonly used molecular markers are given. The data on the varieties with the most effective STB resistance genes are presented: Bulgaria 88, Oasis, Sullivan, Veranopolis, Chinese spring, Tadinia, Flame, Hereword, Curtot, Tonic, Kavkaz-K4500, Arina, Riband, Balance, Apach. Promising markers, mainly microsatellites, or simple sequence repeats (SSR), are recommended for use in breeding for T. aestivum L. resistance to septoria blight. It is technically difficult to use fragment length polymorphisms (AFLP) as molecular markers of resistance genes of cultivated plants. As new resistance genes are discovered and mapped, information about the possibility of pyramiding Stb genes in the breeding material is being updated. The combination of several genes in the genome increases the stability of the variety, since the activity of the genes against STB is manifested at different stages of plant development. The use of known genes in marker-mediated wheat selection and the search for new resistance genes will allow to obtain stable yields with minimal use of pesticides.