A historical review of the formation and development of scientific farming in Siberia is given. The main achievements of the leading research institutes of the region are highlighted. The contribution of Siberian scientists to the development of scientific farming, including its constituent directions, is shown. It presents almost 100 years of development of research on agriculture - from the Omsk experimental farm (XIX century), agricultural experimental stations - to modern federal scientific centers. The evolution of farming from individual crop cultivation techniques to the formation of zonal and then adaptive-landscape farming systems and modern agro-technologies is demonstrated. Examples of completed scientific developments on farming, ready for implementation in production, the number of which runs into thousands, are given. Implementation of knowledge-intensive agro-technologies guarantees grain production in Siberia up to 25-30 million tons, sustainable development of farming, stable food supply and high return on non-renewable resources. The evolution of plant protection from individual methods against the most dangerous pests to the formation of integrated systems that take into account the phytosanitary state of agrocenoses and agro-ecological conditions of agricultural crops cultivation is shown. The most important techniques to optimize the relationship between the crop and pests are the selection of plant species and varieties resistant to their effects, optimization of mineral nutrition of plants and sowing dates. At present, the development of scientific farming is associated with the design of adaptive-landscape farming systems, selection and support of agro-technologies, rational use of land resources, improvement of approaches to the conservation and reproduction of soil fertility, soil treatment systems, fertilizers and integrated plant protection, formation of adaptive crop rotations, research of technological and biological processes in the cultivation of crops, use of GIS-technologies, remote sensing, and the use of remote sensing.

The paper presents the results of research conducted to assess the development of farming systems in the Prienisei Siberia over a long period of time as a basis for identifying factors determining crop productivity, and as an information basis for forecasting crop yields by 2030. The work provides information covering more than two hundred years of land practice in the Prienisei Siberia. It was found that in all the periods of agricultural production, each of which corresponds to a certain farming system, the trend of yield change was positive. Each successive period was shorter than the previous one, with yields increasing significantly. The period of extensive technologies application in farming lasted more than 140 years. At this time, yields increased by 0.0018 t/ha per year. The period of use of the ordinal (low-intensity) farming system lasted approximately 50 years. The yields then increased by 0.0204 t/ha per year. During the period of intensification of the farming system, which took about 30 years, the yields increased by 0.0212 t/ha per year and reached about 2.1 t/ha by the end of the period. The period of formation of high-intensity farming system is the shortest, characterized by the highest productivity growth rates – 0.0639 t/ha per year. If this trend is maintained, the long-term average yield of 3.0–3.5 t/ha will be reached approximately by 2030. For each soil-climatic zone it is advisable to have its own, original farming system, developed taking into account soil-agrochemical properties, landscape and climatic peculiarities, characterized by more in-depth differentiation of individual working area. It should present digital technologies using modern electronic means of informatization, geoinformation systems, remote methods and means of technological processes control, aerial and space methods of agro-ecosystems diagnostics.

The results of improving potato cultivation technology by increasing the efficiency of plant protection system in the conditions of production experience are presented. The study was conducted in 2021–2023 on the lands of the OOO "Druzhba-2" (Bryansk region, Zhiryatinsky district). Two potato varieties of the first reproduction, Red Scarlett (Netherlands) and Gala (Germany), were the object of the study. The experiment scheme included two variants: 1) traditional potato protection system adopted in the farm (control); 2) improved protection system. Both protection systems are aimed at the prevention of pests (Colorado potato beetle, snap beetle larvae), weeds (cleavers, black nightshade, purple deadnettle, lamb's quarters, wild camomile, annual bluegrass, wind bent grass) and diseases (early blight, late blight, silver scurf, rhizoctoniose, various types of rots, pythium). Soils of the sites are sod-podzolic. The forecrop is winter rapeseed. The total area of the production experiment was 120 ha (60 ha under each variety). Potatoes were grown under intensive technology with conventional tillage system on a 75 × 28 cm pattern. Planting rate is 50 thousand tubers per 1 ha. The technology is designed to obtain tuber yields of 40–60 t/ha. Experimentally it has been established that the application of the improved technology with the use of a new plant protection system for the farm contributes to an increase in plant stand density and their safety at the time of harvesting by 2.5–3.0%, increase in the number of tubers from one plant by 7.5–10.0%, average weight of one tuber – by 7–11%, weight of tubers from one plant – by 14.5–23.0%, yield – by 17–26%, marketability – by 12.5–14.5%, marketable yield – by 32–45%, conditional net income – by 16.2–24.5%.

Growth-stimulating properties of Bacillus amyloliquefaciens B-11265 and Bacillus aryabhattai B-13192 strains were studied, and the possibility of their co-cultivation for activity intensification was evaluated. The strains under study were found to be able to synthesize indolyl-3-acetic and gibberellic acids, siderophores: B. amyloliquefaciens B-11265 in the amount of 5.23 µg/ml, 1.05 mg/ml, 46.15%; B. aryabhattai B-13192 – 3.07 µg/ml, 0.84 mg/ml, 35.50%, respectively. The research results showed that the strains were biocompatible and did not inhibit each other's development, allowing them to be included in the same consortium. Joint cultivation of the strains showed that the ratio of microorganisms in the nutrient substrate has a significant effect on the yield of the target substances. At the ratio of B. amyloliquefacien and B. aryabhattai 1 : 1, inhibition of indolyl-3-acetic acid synthesis was observed; ratios of 2 : 3 and 3 : 2 led to a decrease in gibberellic acid production. It was found that three (B. amyloliquefacien) to one (B. aryabhattai) was the optimal ratio to increase the yield of the target substances compared to the average values characteristic of individual strains. In this variant, the synthesis of growth-stimulating substances by the consortium was: indolyl-3-acetic acid – 5.31 μg/ml, gibberellic acid – 1.23 mg/ml, siderophores – 51.27%. The results obtained indicate the high potential of the engineered consortium for agricultural applications. However, in order to develop a growth-stimulating preparation based on it, it is necessary to conduct a number of additional studies aimed at investigating the effect of the strain on agricultural crops in laboratory and field experiments.

The results of research on the influence of physiologically active substances on the growth, development and yield of soybean in the conditions of the Middle Volga region are presented. The study of preparations was carried out on the released soybean variety Yuzhanka. The preparations Mival Agro, Vitanol NP (N 10%, P 30%), Vitanol NP (N 30%, P 10%) and NanoSilica were applied at the dosages recommended by the manufacturers. The experiment scheme included four variants of treatments with preparations and control (water). The preparations were applied for pre-sowing seed treatment and spraying of vegetative plants. The growth regulator Mival Agro (seed and plant treatment) provided a 23.1% increase in yield. Treatment with Vitanol NP (N 10%, P 30%) gave an 18.3% yield increase compared to the control. The highest yield was obtained in the variant with Vitanol NP treatment (N 30%, P 10%), which exceeded the control variant by 68.8%. The yield increase when treated with NanoSilicon exceeded the control variant by 29.4%. Application of all physiologically active substances had a positive effect on the growth, development and productivity of soybean plants. Application of Vitanol NP (N 10%, P 30%) had a positive effect on the yield structure parameters such as plant height (527 cm) and lower bean attachment height (15.8 cm). Vitanol NP (N 30%, P 10%) and NanoSilica preparations contributed to the increase in the number of beans (18.1–18.2 pcs). Also, the studied preparations had a positive effect on 1000 seed weight, plant height and height of the lower bean attachment, compared to the control.

The results of evaluation of five breeding varieties of meadow clover (Trifolium pratense L.) in terms of fodder and seed productivity are presented. Breeding varietal populations of P-15, GPF-49-3, GPF-86-3 SGPF-159-3, SGPF-159-3 + SGP-117 of the double-cut type were investigated in comparison with the standard Dymkovsky for 3 years of economic use of grass stands (2020-2022) in a competitive varietal trial of 2019 sowing. The studies were conducted on strongly acidic (рН_KC1 = 4,47) sod-podzolic soils with high content of exchangeable forms of phosphorus and potassium (192.5 and 217.9 mg/kg), low – humus (2.2%). The weather conditions in the years of research were characterized as moderately warm. The average monthly air temperature for April-August amounted to 13.0-15.6 °C with different precipitation supply: in 2020 – excessive, in 2021 – with a slight deficit, in 2022 – close to normal. During the years of research, high winter hardiness of all the studied varieties (more than 80%) was established. The duration of plant development periods in the varieties at mowed and seed use of grass stands was determined. The economic and biological characteristics of the varieties were given, describing the nature of the changes in plant height and foliage, fodder and seed productivity in comparison with the standard Dymkovsky. Two breeding varieties GPF-49-3 and P-15 have been identified based on the results of this research. The winter hardiness of these varieties was 92.6 and 96.9 %, total dry matter yield for 2 years – 21.90 and 21.56 t/ha (+12.7 and 11.0 % to the standard) and the seed yield on average for 2 years – 1.72 and 2.14 kg/ha (+8.9 and +35.4 % to the standard). These varieties are promising for testing in the Volga-Vyatka region and are suitable for state variety trials.

The results of laboratory and field tests of effectiveness of the Tebu 60 protectant, ME (microemulsion) in its pure form and in tank mixtures with Humat+7 and Fitaktiv Vita against anthracnose seed infection and other diseases of lupine are presented. The research was conducted in 2020–2022 in the Bryansk region. The object of the study were the seeds, seedlings and crops of the Michurinsky white lupine variety. In laboratory conditions, biological evaluation of the effectiveness of the Tebu 60 protectant in pure form (rate of consumption – 0.5 l/t) and tank mixtures Tebu 60 + Humate+7 (0.5 + 0.5 l/t) and Tebu 60 + Fitaktiv Vita (0.5 + 0.05 l/t) against anthracnose seed infection was carried out by the number of affected seedlings grown in paper-polyethylene rolls in comparison with the control (without treatment). The tank mixture Tebu 60 + Fitaktiv Vita (100%) showed high biological efficacy against anthracnose. Moreover, this mixture significantly (LSD₀₅ = 0.66) had the least effect on reducing the hypocotyl length of the seedlings (8.8%). The field experiment was laid down in a fourfold repetition. The plot area was 34 m². The seeding rate was 1.0 million germinated seeds/ha. The soil of the site was gray forest soil, humus content – 2.6%. The forecrop was spring cereal crops. Seed dressing with Tebu 60 (0.5 l/t) and tank mixtures Tebu 60 + Humate+7 (0.5 + 0.5 l/t) and Tebu 60 + Fitaktiv Vita (0.5 + 0.05 l/t) were conducted one month before the sowing. The effectiveness of the treatment was evaluated in comparison with the control. On average, the highest biological efficacy against anthracnose seed infection was shown by the tank mixture Tebu 60 + Fitaktiv Vita (98.1%). By the shiny pod phase, the number of affected beans was 20.5% compared to 78.4% in the control. The biological efficacy against fusarium disease and rhizoctonia was 65.9 and 74.4%, respectively. The prevalence of gray rot and white rot on beans was reduced 8 and 11 times, respectively. Seed germination increased significantly (LSD₀₅ = 0.74) by 16.2%, safety of productive plants to harvesting – by 56.5%. Reliable increase in the seed yield was 2.52 t/ha, cost recovery – 64.88 rubles.

The material about modern genealogical structure of the Kostroma breed of cattle, breeding base and kinship relations of cows in the breeding farms of the Kostroma region is presented. Domestic Kostroma breed of cattle, possessing valuable economically useful qualities, accounts for 33.5% in the region. The studies were conducted in three breeding farms and two pedigree breeding units in 2023. Breeding and zootechnical records data were used as the research material. The research methods are zootechnical, population-genetic and analytical with the use of computer programs. Milk productivity of cows at outbreeding (unrelated breeding) and at different degrees of kinship at inbreeding was analyzed separately for all breeding plants and pedigree breeding units in the Kostroma region. Out of 1736 cows of the Kostroma breed studied, 918 (52.9%) were obtained by outbreeding, 818 (47.1%) by inbreeding, including distant – 461 (26.5%), moderate – 310 (17.8%), close – 41 (2.4%) and consanguinity – 6 (0.4%). Outbreeding and distant and moderate inbreeding were mostly used in breeding farms. The level of spontaneous inbreeding was 2.8%. Higher milk yields are inherent to outbred cows from all breeding farms of the Kostroma region, fat and protein content in milk - to outbred cows and the animals with different degrees of inbreeding in the conditions of specific farms. The results of the research indicate higher milk productivity of the cows under outbreeding. The obtained data were used for making plans of selection and breeding work with the Kostroma breed of cattle in breeding farms.

The results of comparative phylogenetic analysis of 52 isolates of bovine pestiviruses of three species circulating among highly productive dairy cattle in Siberia, as well as those present in the samples of commercial fetal serum, transfected cell culture lines and live vaccines on four genes: N^pro, Erns, E1 and E2 are presented. The obtained data were compared with the results of the 5'UTR gene sequencing obtained earlier. The results confirmed the circulation in Siberia of eleven subtypes of BVDV-1 (a,b,c,d,f,g,i,j, k,p,r), three subtypes of BVDV-2 (a,b,c) and one BVDV -3(a). The genetic profiles of the isolates for the 5'UTR, N^pro, Erns and E1 genes matched completely. The exceptions were three BVDV-1a isolates (R/FBS/96, N/MDBK/08 and SA/FBS/08) detected in infected cell cultures that clustered in a clade formed by BVDV-1j reference sequences. The remaining isolates previously shown to have 100% sequence identity of the 5'UTR had nucleotide sequence similarities of the N^pro, Erns, E1, and E2 genes ranging from 96-99%, which also implies that they are closely related. The results of the BVDV-2 isolates sequencing showed a complete correspondence with the genome sequences identified in the previous studies. Comparative sequencing also confirmed the circulation of H Italian-Brazilian pestivirus isolates (BVBD-3a) in Siberia, but all the isolates were divided into two subclades. The first included the isolates isolated from fetal sera and cell cultures, while the second included those isolated from the vaccines and internal organs of diseased animals during outbreaks in farms. The genetic diversity of bovine pestiviruses, their origin and variability are discussed.

Recently, stress has become the most urgent problem of modern animal husbandry. With the industrialization of agriculture, this problem is becoming more and more acute, as a result, livestock enterprises and farms suffer significant economic losses. Throughout its life, the animal is exposed to numerous stressors that have a completely different nature of occurrence, but invariably lead to the same changes in the body. The animal noticeably loses weight, weakens, its resistance to diseases decreases. A review of scientific sources on some types of stress loading on cattle: heat stress, transportation stress, pre-slaughter stress is given. According to many studies, the stress state of an animal depends on feeding and maintenance by 70-80% and only 20-30% on genetics. The body of farm animals is constantly affected by various environmental factors. These include production technology, method of maintenance, density of accommodation, size of groups, microclimate of premises, type and level of feeding, biological full-value of diets, methods of preparation and distribution of feed, quality of drinking water, veterinary and preventive and zootechnical measures (vaccination, sanitary treatment of animals, weighing, castration, etc.). When the above factors change, certain reactions occur in the animal body. The nature of stress can be different: mechanical, physical, chemical, biological, mental. Knowledge of the causes of stress, the patterns of its course, its consequences, outlined in the mentioned scientific sources, will allow rational use of the proposed ways to reduce the adverse effects of various stress factors on animals to preserve their health and high productivity.

The results of studying the process of changing the biopotential of wheat seedlings of the Novosibirskaya 41 variety when exposed to elevated (43 °C) and reduced (8 °C) temperatures are presented. The experiments took place in 2020 and 2022. The automated system "AutoExpI" was used in the research. The data on the dynamics of plant biopotential resulting from identical temperature changes in the environment when the age of seedlings changes were obtained. The mean absolute maximum centered values (U _{max c}) of the biopotential of seedlings aged 10 to 19 days when exposed to elevated and reduced temperatures in 2020 and 2022 were statistically analyzed using Statistica 6 software (Statistica 6). The regression relationships determined during the regression analysis of the biopotential indices under the action of elevated temperature are described with respect to the age of seedlings by second-order polynomials with correlation coefficient R = 0.997 at p = 0.072 for the results of the experimental studies in 2020 and R = 0.986 at p = 0.028 for the data for 2022. For the effect of reduced temperature, quadratic regression relationships were also obtained with a correlation coefficient of R = 0.995 at p = 0.1023 for the 2020 experimental results and R = 0.9998 at p = 0.00023 for the 2022 data. The closeness of the graphical representation of similar regression dependencies was noted, on the basis of which the algorithm was determined and expressions for correction of experimental data obtained in the process of prolonged experimental studies were obtained.