The results of research on the dependence of agrochemical and agrophysical properties of leached chernozem on the system of soil treatment are presented. The work was performed in 2015–2019 in a long-term stationary field experiment in crops of spring soft wheat Siberian Alliance. The soil of the experimental site is medium-powered medium-humus heavy-loamy leached chernozem. Wheat was preceded by clean fallow, green fallow (rape), green fallow (cloverleaf). The following tillage systems were studied: deep moldboard (control), deep combined, minimum combined, minimum moldboard. There is an advantage in nitrate nitrogen content in the soil before sowing, in the phase of tillering and earing of wheat on the preceding cereal fallow (rape) when using the minimum tillage system (in autumn the break crop BDT-3 is planted). The effect of the tillage system on the nitrate nitrogen content in the soil in the phase of bushing of wheat - 15,5%, conditions of the year - 12,9, the interaction of these factors - 20,1% was determined. A positive relationship between the content of nitrate nitrogen in the soil and the amount of mobile phosphorus on the precursor green fallow (rape), r = 0.7118-0.8917 (R = 0.9500), was detected. High P₂O₅ content (from 150 mg/kg and above) was recorded on average for 5 years during the earing phase of wheat - 145.0 to 165.6 mg/kg. Maximum P₂O₅ values were detected for green fallow (rape) under minimum combined and minimum moldboard systems. A significant increase in exchangeable potassium content over the control average for 2015-2019 was observed with the deep combined tillage system - 5.0 mg/kg (NSR₀₅). With the other tillage systems studied, the figures were at the control level. Higher soil pedality coefficients were revealed with a deep moldboard processing system (control) - 2.54, a minimum combined - 2.47, a minimum moldboard - 2.23 according to the predecessor green fallow (rapeseed); for green fallow (melilot) - 2.98 (deep moldboard). With an increase in the pedality coefficient, there was a decrease in the soil bulk density index, r = -0.3499 (R = 0.5760). A tendency was revealed towards a decrease in the soil bulk density to 0.98 g/cm³ according to the predecessor green fallow (rapeseed) with minimization of soil cultivation: combined moldboard and minimum moldboard; with a deep moldboard (control) - 1.02 g / cm³. As a result of the correlation analysis, it was found that with an increase in the soil bulk density, the content of P₂O₅, r = –0.4898, K₂O, r = –0.2530, decreases.

The results of research on the effect of crop protection agents on tomato and soybean crops under Primorye conditions are presented. The study of preparations was carried out on zoned varieties of tomato Novichok and soybean Primorskaya 86. The effectiveness of different combinations of biological preparations based on Bacillus subtilis strains Bactophyt, Gamair and growth regulators Zircon, Mival-Agro was evaluated. The preparations were used for seed treatment and spraying of vegetative plants. Complex treatment with Bactofit provided a 3.9% reduction of septoriosis manifestation on tomato, the biological efficiency was 22.1%. Growth regulator Mival-Agro (seed and plant treatment) provided 36.8% effectiveness against late blight. The highest tomato yield was observed with the treatment of seeds and plants with Gamair (20.0 t/ha) with an increase of 4.9 t/ha compared to the control. The maximum efficiency (29.7%) against septoriosis on soybeans was recorded in the variant Gamair + Mival-Agro. The biological efficacy against peronosporosis ranged from 21% (Gamair complex treatment) to 25.4% (Bactofit + Mival-Agro). The application of all preparations had a positive effect on the growth and development of soybean plants. The largest increase in plant height in the phase of full ripeness was noted in the variant with the use of Bactofit and Mival-Agro (73.1 cm), in the control - 61.8 cm. Thousand-kernel weight increased with certainty by 13.5 – 22.0% in comparison with the control (176.2 g) after the usage of biopreparations and growth-regulating chemicals. High yield was achieved in the Bachtophit + Mival Agro variant (2.7 t/ha), in comparison the control was 1.8 t/ha, the addition amounted to 0.9 t/ha.

Adaptive properties of spring barley in the conditions of the Kuznetsk depression have been studied. The objects of the research are five medium-ripening breeding lines of spring barley. The breeding lines were studied in a nursery of competitive varietal trials in 2016-2020. The soil of the experimental plot is leached chernozem, heavy loam with a medium granulometric composition. The repetition was fourfold, the seeding rate (optimal for spring barley in the cultivation zone) 450 pcs / m². The sowing dates were April 29 - May 5, the harvesting was done with a Sampo 130 combine at the stage of full ripeness of spring barley samples (August 12-15). The experiment was carried out in comparison with the standard cultivar Biom. The parameters of environmental plasticity (bj), stability (S2dj), the environmental conditions index (Ij) were calculated according to the method developed by S.A. Eberchart and W.A. Russel, which examines the positive response of the genotype to the improved growing conditions. Under contrasting growing conditions of spring barley in the years of research, it was found that the environment factor had a significant influence on the yield - 82.6%, the share of genotype influence was 1.4%. The average yield over the years of the study of spring barley in the nursery of competitive variety trials was 5.51 t/ha, the maximum average yield has a breeding line KM-198/11 - 6.45 t/ha. Genotypes with the highest adaptive properties were identified: KM-198/11 (bi = 0.24, Si2 = 2.59), Nutans 12/16 (bi = 0.24, Si2 = 2.18) with yield variability of 25.9-29.2% (Biom standard - 46.1%). The medium-maturing selection line of spring barley KM-198/11, having above-average adaptive properties, is prepared for submission for state variety testing in 2021 as the variety Kuzbass Jubilee. The main advantages of the variety were revealed: high drought tolerance, resistance to lodging and blight, high productivity (6.45-8.50 t/ha), large grain size (53.2 g), medium reaction to stress factors (reduced productivity). Grain crude protein content was 13.6-14.1%, the film content 8.4%, the natural weight 620 g/l.

The paper presents results of research into breeding of the new soybean cultivar Gorinskaya by the methods of hybridization and individual selection. Soybean varieties SibNIIK-315 (female parent) and Fiskebi V (male parent) were used as a starting material. The studies were carried out in the forest-steppe zone of the Western Siberia (Novosibirsk region). Soybean Gorinskaya belongs to the Manchu subspecies. The plants have a light brown (reddish) pubescence of the stem, leaves, beans. The growth pattern and the type of apex is intermediate, the number of branches is 1–3, the angle of branching is 20–30 degrees, the bush is compressed. The height to the first branch is 6–10 cm, the attachment height of the lower pod is 10–13 cm. The beans are distributed evenly throughout the plant. The length of the stem is 55–75 cm, the number of internodes on the stem is 12–15. The inflorescence is a small-flowered raceme of 3-5 flowers. The corolla is purple in color. The pods are slightly curved with a pointed tip; when ripe, they acquire a brown color. The seeds are elongated-oval, greenish-yellow in color, without pigmentation. The seed hilum is brown. The cultivar is the grain variety for use. The yield in the competitive variety testing reached 29.4 c / ha. The mass of seeds per plant is 10–12 g, the mass of 1000 seeds is 150–160 g. The number of seeds per pod is 2-3; the average number of pods per 1 productive node is 2-3. The protein content in seeds is 35-38, fat – 17-19%. Duration of the vegetative period is 100-105 days. The cultivar is medium resistant to diseases, cold, drought, and soil salinity. In 2018, the Gorinskaya soybean variety was included in the State Register of Breeding Achievements Approved for Use in the Russian Federation for the East Siberian region.

One reliable way to restore eroded soils and technogenic land is to reclaim it by sowing perennial grasses. Samples of reed canary grass suitable for reclamation of coal dumps in the Kemerovo region have been studied and identified. Experimental work was carried out in the Tomsk region (5811 N, 8300 E) during the period of 2017-2020. The research subjects are represented by samples of reed canary grass taken in 2016. A nursery of study samples suitable for reclamation of coal dumps was laid in 2017 with six numbers - KM-1, KM-2, KM-3, KM-4, KM-5 (Tomsk region), the variety Vityaz (Tomsk region) was used as a standard. The soils of the experimental plots were sod-podzolic acidic loamy sandy loam and loamy with a humus content of no more than 2% in the arable horizon. When evaluating the samples, the main attention is paid to studying the traits and properties that limit their cultivation under taiga conditions: grass density, plant height, foliage, winter hardiness, lodging resistance, seed productivity, green mass yield, resistance to the most common diseases. Over a 3-year study of selections of reed canary grass based on a set of the main economically important traits, the KM-5 sample has been identified. This specimen has a complex of valuable features in creating an adaptive variety suitable for reclamation of coal dumps (foliage - 61.4%, green matter yield - 38.5 t/ha, dry matter - 11.9, seeds - 0.21 t/ha). The selected number has annual reliable additions to the standard in terms of green matter yield of 8.5 t/ha, dry matter yield of 3.3, and seed yield of 0.04 t/ha. Samples KM-1, KM-5 showed high resistance to these diseases: helminthosporiosis - 6.0-6.1%, septoriosis - 2.6-4.2%.

The results of the use of entomophages and biological products to regulate the number of cabbage pests (cabbage moth Mamestra brassicae L., cabbage butterfly Pieris brassicae L., turnip butterfly Pieris rapae L., diamondback moth Plutella xylostella L.) are presented. The study was carried out in the Primorsky Territory in 2018-2020. The efficiency of Trichogramma ussuricum Sorokina applications was assessed on cabbage varieties. The effectiveness of the entomophage against the cabbage moth varied from 33.3 to 66.6%, against the turnip butterfly - from 32.6 to 70.2%. In field experiments the effectiveness of biological products Fitoverm EC (0.09 l/ha), Akarin EC (1.6 l/ha), Proclaim WG (0.3 kg/ha), Bitoxibacillin (10 l/ha), Bitoxibacillin P (2 kg/ha), Lepidocid SC (2 l/ha), Lepidocid P (2 kg/ha) against diamondback moth are studied. Cabbage plants were sprayed with the preparations once. Pest counts were carried out before treatment and after treatment on the 5th, 10^th and 15th day in accordance with the approved methods. Bioinsecticide Proclaim showed a high efficiency of 93.0-100% on the 5-10th day. The effectiveness of preparations based on aversectin C and avertin N was 65.0-88.6%. Using the biological product Bitoxibacillin and Lepidocid a decrease the number of diamondback moth relative to the control by 61.2-97.5 и 65.0-78.0% was registered.

The results of studies on productivity and genotypic structure of cows of the leading lines of Sibiryachka cattle breed, associative links of CSN3, BLG, LALBA, LEP genotypes with economically important traits are presented. Comparative evaluation showed that Reflection Sovering bull cows had the highest milk yield of 6851 kg, fat content of 4.05% and protein content of 3.15%. The Siberian bull lines Frank 937, Uragan 27 and Kursa 1949 which are being shaped are inferior to them in milk yield, fat and protein content with values of 5246-5504 kg, 3.92-3.94%; 3.10-3.12% respectively. The genotypic structure of the herd and the leading lines is identified. The Vis Back Aydiala bull line is characterized by a higher frequency of CSN3AA and LEPTT genotypes by 18.2 and 11.0%, in comparison with the Reflection Sovering line. For other genotypes, the differences do not reach the confidence threshold. The average level of homozygosity for the genes studied varies from 51.2% to 73.4%. The highest homozygosity was found for the CSN3 gene in the Vis Back Aydiala line at 79.6%. The number of effectively acting alleles is 1.66-1.72; the degree of genetic variability is 40.2-42.7%. The cows with CSN3AB genotype had 544.0 kg higher milk yield than homozygous animals for the A allele (p <0.05). The highest milk yield was observed in BLGAA animals - 6790.1 kg, which is 947.2 kg higher than in cows with the alternative BLGВВ genotype (p < 0.01). Animals with the LEPCC genotype outperformed LEPTT cows in milk yield by 718.7 kg. No priority genotypes were identified for the LALBA gene. Also, no connection has been established between genotypes and the quality indicators of milk.

The level of expression of anti-inflammatory cytokines NF-kB, IL-6, IFN-y, Caspasa-3, FC in chickens in the lungs and intestines during the modeling of infectious bronchitis in chickens was studied. To simulate coronavirus pneumonia, the vaccine was administered individually, 10 doses per head orally. The chickens of the 1st experimental group were fed with the Lyumantse preparation at the rate of 3 kg / t of feed, the 2nd experimental group received the Glitsevir drug at the rate of 200 μg / 0.3 ml per head. The chickens of the control group did not receive the preparations. It was revealed that antiviral drugs in the experimental groups suppressed the destruction of epithelial cells in the intestine. This may not always be an indication of a positive character, as in the case of apoptosis, not only the intestinal cells affected by the virus particles but also healthy cells are destroyed. There was a decrease in the number of active macrophages in the intestines of the experimental groups relative to the control. The amount of interferon produced was also below the control, which indicates a decreased activity of the immune system. A higher pro-inflammatory activity in the respiratory system of chickens was detected when Glicevir was used. It consists of increased expression of IL-6, interferon-gamma, macrophage receptor to Fc antibody fragments and inflammatory regulatory factor NF-kB genes compared to Lumantse with anti-inflammatory activity, but also compared to untreated control group chickens. It is concluded that it is possible to predict the risk of an exacerbation of an infectious process in the lungs against the background of a local decrease in the viral load in the intestine. An integrated approach is needed in the treatment of coronavirus infections, including either systemic antiviral drugs or anti-inflammatory drugs.

The dynamics of digestive diseases of bacterial etiology among cattle are presented. Statistical, laboratory and annual average data on registered animals with this pathology, mortality and forced slaughter of livestock were analysed for the period 2016-2020. It has been found that digestive diseases of bacterial etiology are prevalent both in young cattle (53.7% to 61.4%) and in adult cattle (43.1% of the total number of diseased animals). The following pathogens were identified on the basis of bacterial tests on biological material: enteropathogenic E. coli (37.8% of the total number of diseased animals) and group D streptococci, E. Faecalis (15.1%). The rest of the herd had associated infections (19.2%) and non-contagious digestive diseases (27.9%). As a result of this monitoring, a high incidence of digestive diseases among cattle in livestock farms in the following districts of the Trans-Baikal Territory was recorded: Akshinsky, Krasnochikoysky, Kyrinsky, Nerchinsky, Olovyaninsky, Priargunsky, Khiloksky, Shelopuginsky, Aginsky. The lowest prevalence of cattle diseases in organizations of various forms of ownership has been recorded in peasant farms in the region (6.8-10.9%). The average number of registered animals with this pathology is recorded in agricultural organizations (26.9-37.6%). A high percentage of these diseases were recorded in households (62.8%). When farms follow the organization of therapeutic and prophylactic measures, housing and feeding hygiene rules, it is possible to ensure the safety and productivity of livestock, high quality and safety of livestock products and to prevent bacterial diseases of the digestive organs.

The problem of food contamination by heavy metals due to their accumulation in biosystems as a result of anthropogenic influences has been studied. The results of cadmium, lead, iron and manganese in cattle from the Novosibirsk Region and the Altai Territory are presented. The purpose of the study was to assess the impact of the ecological and geographical factor on the heavy metal content in the lungs of Hereford bulls. Lung samples were taken from 31 clinically healthy Hereford bulls aged 16-18 months. Sample preparation was carried out in accordance with GOST 26929-94 and 30178-96. The chemical composition of the samples was determined by atomic absorption spectrometry with flame and electrothermal atomization. The median values of the content of cadmium, lead, iron and manganese in the lungs of bull-calf were in the range of 0,01-0,12; 0,07-0,11; 70,9-89,1 and 0,3-0,4 mg/kg. The phenotypic variability of the level of iron and manganese was relatively low, while that of lead and cadmium was high. The dispersions of lead and cadmium levels in the animal groups studied were homogeneous, manganese and iron variants were heterogeneous. The influence of the ecological and geographical factor is characteristic only for cadmium levels. For manganese, iron, lead and cadmium the calculated reference ranges are: 0-1.12; 0-148.1 and 0-0.170 mg/kg respectively. They can serve as a guideline for Hereford cattle bred in the territories of Krasnozersky, Novosibirsky and Maslyaninsky districts of the Novosibirsk region and the Tselinny district of the Altai Territory.

The possibilities and feasibility of using the Bayesian network of trust and logistic regression to predict the content of nitrate nitrogen in the 0-40 cm soil layer before sowing have been investigated. Data from long-term multifactor field experience at the Siberian Research Institute of Farming and Agricultural Chemization of SFSCA RAS for 2013-2018 were used to train the models. The experiment was established on leached chernozem in the central forest-steppe subzone in 1981 in the Novosibirsk region. Considering the characteristics of the statistical sample (observation and analysis data), the main predictors of the models affecting nitrate nitrogen content in soil were identified. The Bayesian trust network is constructed as an acyclic graph, in which the main (basic) nodes and their relationships are denoted. Network nodes are represented by qualitative and quantitative plot parameters (soil subtype, forecrop, tillage, weather conditions) with corresponding gradations (events). The network assigns a posteriori probability of events for the target node (nitrate-nitrogen content in the 0-40 cm soil layer) as a result of experts completing the conditional probability table, taking into account the analysis of empirical data. Two scenarios were analyzed to test the sustainability of the network and satisfactory results were obtained. The result of the logistic regression is the coefficients characterizing the closeness of the relationship between the dependent variable and the predictors. The coefficient of determination of the logistic regression is 0.7. This indicates that the quality of the model can be considered acceptable for forecasting. A comparative assessment of the predictive capabilities of the trained models is given. The overall proportion of correct predictions for the Bayesian confidence network is 84%, for logistic regression it is 87%.

The process of daily variation in tomato stem diameter is examined in order to justify the use of this parameter to control drip irrigation. Changes in the size of individual plant parts depend on the provision of water, light, heat and nutrients to the production process. Therefore, such plant parameters as leaf temperature, xylem flow rate, fruit and stem diameter can be indicators of availability of necessary resources. The research was carried out in Novosibirsk region in June - September 2020. The value of the range of daily variations in stem diameter, which has a close relationship to relative soil moisture, was used as an indicator of plant water stress. The source of the information is the results of measurements of soil moisture and stem diameter growth of tomato. Experiments to assess the effect of water deficit on stem parameters were carried out on a plant set out in the open ground separately from the rest. Artificial water stress conditions were created by watering once a week. Data were collected using a PM-11z phytomonitor, soil moisture and stem diameter growth sensors. The results of measurements were processed in Microsoft Office Excel program. It was found that the range of daily fluctuations of stem diameter growth depends on moisture availability. When soil moisture is below 30%, the plant experiences water stress and the range of stem diameter fluctuations increases. The maximum growth in stem diameter was observed at 7-10 a.m. and the minimum at 13-15 p.m. local time. The difference between the maximum and minimum of the daily stem diameter increase characterizes the range of the daily stem diameter difference, which correlates closely with soil moisture. The correlation coefficient between them is 0.72. The limit for the daily stem diameter difference is 0.025 mm at 30% soil moisture. If the actual value of this parameter exceeds the limit value, the irrigation system can be activated. The implementation of this approach makes it possible to automate the irrigation process and to take into account the indicator that signals water stress of the plant.

The article covers the main directions of joint work of scientific-research institutions (SRI) of agricultural profile in the Siberian region and Siberian Scientific Agricultural Library (SibSAL) functioning within the structure of the Siberian Branch of VASKHNIL (Russian Agricultural Academy) since its establishment in 1969 up to the reorganization period of science reform (2013-2021). The specificity of library and information activity of departmental library and multilevel model of interaction with institutes to satisfy information needs of scientists and specialists of agrarian research institutes in pre-reform period is presented. The results of monitoring the state of library and information activities in the institutes that have influenced the development of the new concept of interaction with SRI are presented. Based on the analysis of reorganization processes, a new structure of scientific organizations of the agricultural profile of Siberia is presented, which is the basis for the development of a new paradigm of interaction between research teams and the Siberian Scientific Agricultural Library, which received the status of a branch of the State Public Scientific and Technical Library of the Siberian Branch of the Russian Academy of Sciences as a result of the reorganization. The analysis carried out in the conditions of reform and reorganization changes on the basis of a questionnaire shows new trends in expanding the range of interests of scientists to the possibilities of the information potential of the SibSAL as the largest branch library in the region. In the context of the change of management and the destruction of organizational and functional ties of agricultural research institutes that have become part of the regional centers of the SB RAS, the orientation to the development and strengthening of communications with the central academic libraries of the regions and the restoration of interaction with libraries of agricultural education on a new basis is noted. The definition of the system structure of agricultural research institutes and educational institutions of the region within the new boundaries of departmental relations of the Ministry of Agriculture, the Ministry of Science and Higher Education will allow establishing the vector of interaction of the SibSAL as a system-forming link. This will enable the development of the main functional areas of library and information activities in the Siberian region.

The immunogenicity of a virus vaccine against viral pneumoenteritis in young cattle was studied in laboratory animals. The results of the selection of optimal vaccine strains to study the effects of different inactivants on infectious rhinotracheitis virus, viral diarrhea, parainfluenza-3, respiratory syncytial virus, rotavirus and coronavirus are presented. The accumulation of avirulent vaccine virus strains was carried out using established virological methods on transplanted cell cultures of MDBC (calf kidney cells) and SPEV (fetal pig kidney cells). Theotropine and formalin were used as inactivating agents to develop inactivation regimes for vaccine strains - components of the experimental vaccine. The antigenic activity of attenuated strains of infectious rhinotracheitis virus, viral diarrhea, parainfluenza-3, respiratory syncytial virus, rotavirus and coronavirus on white mice and calves and the level of specific antibodies in the sera of guinea pigs immunized with vaccines using different adjuvants were studied. Avirulent strains of viruses were used in the design of a new virus vaccine against viral pneumoenteritis: infectious rhinotracheitis (IBR-VBF-VHAVM No. 404); diarrhea (VDVBF-VHAVM No. 406); parainfluenza-3 (PG-VBF-VHAVM No. 403); respiratory syncytial virus (RSV-VBF-VHAVM No. 405); rotavirus (RTV-VBF-VHAVM No. 401) and coronavirus (CV-VBFVHAVM No. 407). The selected vaccine strains are non-reactive and induce active production of antiviral antibodies in sufficiently high titres in both laboratory animals (white mice) and farm animals (cattle). The most effective inactivating agents are 0.1% theotropine and 0.2% formalin. Two types of oil-based adjuvants, ISA 15 and ISA 25, were used to select the optimal adjuvants for the design of the virus vaccine. Adjuvant ISA 15 was used at 15% of the antigen quantity, ISA 25 at 25%. The adjuvant IZA 15 at a concentration of 15% is the optimum adjuvant for the preparation of an experimental virus vaccine against viral pneumoenteritis in young cattle.