AGRICULTURE AND CHEMICALIZATION
The work presents the results of comparative field tests on effectiveness of insecticides and bioinsecticides and their tank mixtures for potatoes against the potato ladybird and the Colorado beetle in the South of the Far East. Effectiveness of preplanting and vegetation treatment of potatoes with preparations was observed. The study, conducted in 2012–2014 on the plants of the recognized Yantar potatoes variety, showed high biological efficiency of preparations from the neonicotinoid class of Prestige and Cruiser in the rates of 1.0 l/t and 0.2 l/t by pre-planting treatment of potato tubers. The Prestige and Cruiser provided long-term protective effect against the Colorado potato beetle and the potato ladybird, which eliminated the need for treatment in the growing season of the crop. The use of these insecticides caused productivity increase of potatoes by 7.5 and 8.4 t/ha. For vegetation treatment the preparation Decis Extra showed the highest and the most long-lasting effect. On the 28th day after the treatment, its effectiveness accounted for 84.4–90.3%. Protective effect of bio-insecticide Phytoverm lasted for 14 days. Double treatment of potatoes with this bio-insecticide restrained the number of pests below the threshold number until the end of the growing season. During the research it was found that the joint use of Phytoverm with 3 times lower rates of the insecticides Decis Extra and Konfidor provided a significant reduction in the number of the potato ladybird and the Colorado potato beetle and the preservation of 39.3-41.6% of potato productivity.
PLANT GROWING AND BREEDING
The work presents the results of the research into studying technological qualities of the grain of spring common wheat, variety Siberian Alliance, depending on the soil tillage system in grain-fallow crop rotation. The research was conducted in the northern forest-steppe of Kuznetsk Depression in the experimental plot established in 1974, in a fourcourse grain-fallow crop rotation (fallow – wheat – peas – pure barley and barley intercropped with melilot). Various soil tillage systems were applied: deep moldboard, deep combined, minimum combined, minimum moldboard. The study of grain quality took place on crops of spring common wheat by three preceding crops on bare fallow and greenmanured fallow with rape and melilot. The soil of the experimental plot was characterized as leached chernozem, medium-textured, medium humus, heavy-loam. The humus content in an arable layer was 8.2%. Crops of spring common wheat were sown with all types of soil tillage systems by the multifunction tillage and sowing machine Tom’5.1. Optimum conditions for obtaining grain of spring common wheat of high quality were formed on green-manured fallow with intercropping of melilot and rape by moldboard and minimum combined soil tillage. On average, the mass of 1000 grains amounted to 37.0-37.4 g, in 2017 – 41.0 g, natural weight – 725-751 g/l, hardness – 80-81%, content of crude gluten – 32-33%, mass fraction of protein – 15.2-15.3%. Positive correlations between indices of grain quality, r = 0,40700,6594 were established. According to the State Standard Specification P 52554-2006, cultivar of common spring wheat Siberian Alliance confirms its belonging to the strong wheat type by the quality indices obtained.
The work presents the results of evaluation of 150 varieties of sowing peas (Pisum Sativum L.) of different ecological and geographical origin that have not lost their breeding value in recent years by a number of economically valuable signs from the world collection of The N.I. Vavilov All Russian Institute of Plant Genetic Resources. The purpose of the research was to identify promising varieties of sowing peas from the collection to create modern varieties of peas for grain use. The experiment was conducted in 2014–2016. on leached chernozem of the northern foreststeppe of Western Siberia. The weather conditions during the years of research were contrasting. So, in May and July of 2014, precipitation was above the average rainfall, which caused the formation of tall pea plants, especially in the varieties of leafy morphotype. In 2015, the air temperature was above the long-term average annual rate with uneven precipitation. In 2016, the air temperature in May generally corresponded to the long-term average annual rates, but August was characterized by precipitation deficit. The maximum yield for the years of research was noted in the varieties of leafy morphotype group: Bogatyr (625 g/m2), Kemchug (510 g/m2), and the group with the modified morphotype: Az-318 and Orel (480 g/ m2). Varieties with the modified morphotype Az-318, Sprut-2, Orel, Demos, Yamal proved to be highly resistant to lodging and easy to harvest, due to being adapted to the soil and climatic conditions of Western Siberia. The correlation analysis conducted in 2016 revealed a reliable positive relationship (r = 0.63) between the mass of 1000 grains and the yield. A closer positive relationship was found during all the years of the study between the yield and the number of peapods on the plant (r = 0.48–0.67). A negative dependence was observed between the length of the plant and the yield.
FODDER PRODUCTION
During the research, non-traditional fodder crops that have high fodder productivity and adaptability to natural and climatic conditions of Trans-Baikal Territory were studied. The results of field and laboratory studies for 2014–2016 are presented on the cultivation of annual poaceous fodder crops – feed millet, Sudan grass and barley in single-crop sowings, as well as mixed with high-protein spring rape, in the forest-steppe zone of Trans-Baikal Territory. The research was conducted on meadow-chernozem mealycarbonate soil. Fodder crops were assessed by their adaptability to growing conditions and by a set of economically valuable characteristics. The possibility of increasing productivity and quality of fodder of poaceous agrocenoses by means of cabbage crops was established. Joint sowings of annual fodder crops: feed millet + spring rape, Sudan grass + spring rape provided the maximum collection of green mass – 24.4-25.9 t/ha, dry matter – 3.55-3.78 t/ha, feed units – 3.17-3.30 t/ha, digestible protein – 506-545 kg/ha, gross energy – 37.3-39.3 GJ., with a high supply of digestible protein to a feed unit – 160-165 g/k per unit. Mixed sowings of Sudan grass and feed millet with spring rape increased collection of feed units by 22.3%, digestible protein – by 86.8%, gross energy – by 18.5% compared to single-crop sowings. Barley, when cultivated under the same conditions, was inferior in productivity to feed millet and Sudan grass in single-crop sowings by 1.2-1.7 times, in mixed sowings – by 1.0-1.5 times.
ANIMAL HUSBANDRY ABD VETERINARY SCIENCE
The use of genetic markers in addition to traditional methods of animal selection with desirable genotypes allows to increase the share of animals with high productivity in the next generations and ensures improvement of breeding efficiency. Genetic features of the Prikatun type of the Gorno-Altay semi-fine wool breed of sheep and the white downy goat breed in the Republic of Altay were studied by the method of immunogenetic and molecular genetic analysis. The frequency of antigenic factors was identified and the index of genetic similarity between goats and sheep and their separate herds was calculated. Gene polymorphism of β-lactoglobulin (BLG) and calpastatin (CAST) was revealed by the method of DNA diagnosis. Population and genetic characteristics of the herds was studied by the genes specified. Associative genotype relation to productivity and quality of the produce obtained was analyzed. The index of genetic similarity between the goats and the sheep was at the level of 0.713, between the separate herds of the goats the index was 0.861. The ratio of genotypes in the BLG gene determined by PCR analysis in the white downy goats was S1 S1 – 16.1%; S1 S2 – 50.6%; S2 S2 – 33.3%. In the Prikatun type two genotypes were identified in this gene: BB with the frequency of 59.2%, and AB – 40.8%. Two different alleles were identified in the CAST gene of sheep (M and N). The genotype MM was the predominant variant in the CAST sheep gene, whose frequency was 88%. The frequency of occurrence of animals with NN genotype was 1%, MN – 11%. It was shown that the gene equilibrium in the herds was not broken, χ2 = 0.931. It was noted that heterozygous goats (S1 S2 ) by BLG gene had a higher live weight by 0.30-0.61 kg compared to other variants of BLG gene (p<0.05). It was also found that lambs with genotype MM of the CAST gene had a higher live weight by 5.5 kg than MN heterozygotes (p< 0.01). However, this difference was not revealed in other age and sex groups of animals.
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